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ZFIN ID: ZDB-PUB-051114-13
The zebrafish bcl-2 homologue Nrz controls development during somitogenesis and gastrulation via apoptosis-dependent and -independent mechanisms
Arnaud, E., Ferri, K.F., Thibaut, J., Haftek-Terreau, Z., Aouacheria, A., Le Guellec, D., Lorca, T., and Gillet, G.
Date: 2006
Source: Cell death and differentiation 13(7): 1128-1137 (Journal)
Registered Authors: Gillet, Germain, Le Guellec, Dominique
Keywords: apoptosis, Bcl-2, gastrulation, Nr-13, somites, zebrafish
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Apoptosis/physiology*
  • Blotting, Western
  • COS Cells
  • Cercopithecus aethiops
  • Cloning, Molecular
  • Gastrula/cytology
  • Gastrula/metabolism
  • Gastrula/physiology*
  • Gene Expression Regulation, Developmental
  • In Situ Hybridization
  • Microscopy, Confocal
  • Molecular Sequence Data
  • Oligonucleotides, Antisense/genetics
  • Proto-Oncogene Proteins/genetics
  • Proto-Oncogene Proteins/metabolism
  • Proto-Oncogene Proteins/physiology*
  • Proto-Oncogene Proteins c-bcl-2/genetics
  • Proto-Oncogene Proteins c-bcl-2/metabolism
  • Proto-Oncogene Proteins c-bcl-2/physiology
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Homology, Amino Acid
  • Somites/cytology
  • Somites/metabolism
  • Somites/physiology*
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • Transcription Factors/physiology
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/physiology
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Zebrafish Proteins/physiology*
PubMed: 16282981 Full text @ Cell Death Differ.
FIGURES
ABSTRACT
Although the role of the b-cell lymphoma (Bcl)-2 family of apoptosis inhibitors is well documented in tumor cells and tissue morphogenesis, their role during the early development of vertebrates is unknown. Here, we characterize Nrz, a new Bcl-2-related inhibitor of apoptosis in zebrafish. Nrz is a mitochondrial protein, antagonizing the death-accelerator Bax. The nrz gene is mainly expressed during gastrulation and somitogenesis. The knockdown of nrz with antisense morpholinos leads to alterations of the somites, correlated with an increase in apoptosis. In addition, earlier during development, in the zebrafish gastrula, nrz knockdown results in an increase of snail-1 expression at the margin and frequent gastrulation arrest at the shield stage, independently of apoptosis. Together these data suggest that Nrz, in addition to its effect on apoptosis, contributes to cell movements during gastrulation by negatively regulating the expression of Snail-1, a transcription factor that controls cell adhesion.
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