ZFIN ID: ZDB-PUB-051019-5
Zebrafish KLF4 is essential for anterior mesendoderm/pre-polster differentiation and hatching
Gardiner, M.R., Daggett, D.F., Zon, L.I., and Perkins, A.C.
Date: 2005
Source: Developmental dynamics : an official publication of the American Association of Anatomists 234(4): 992-996 (Journal)
Registered Authors: Daggett, Dave, Perkins, Andrew, Zon, Leonard I.
Keywords: BMP4, Cap1, CatL, hatching gland, anterior mesendoderm, polster, zebrafish, zKLF4
MeSH Terms: Animals; Cell Differentiation/physiology*; Endoderm/metabolism; Endoderm/physiology*; Gene Expression Regulation, Developmental* (all 11) expand
PubMed: 16222715 Full text @ Dev. Dyn.
FIGURES   (current status)
ABSTRACT
Gene knockout studies of Kruppel-like factors (KLFs) in mice have shown essential roles in organogenesis. A screen for KLF family members in zebrafish identified many KLFs. One of these, zebrafish KLF4 (zKLF4) is the homologue of neptune, a Xenopus laevis KLF. zKLF4 is expressed from approximately 80% epiboly a patch of dorsal/anterior mesendodermal cells called the pre-polster and, subsequently, in the polster and hatching gland. Here we investigate the function of zKLF4 using morpholino-based antisense oligonucleotides. Knockdown of zKLF4 resulted in complete absence of hatching gland formation and subsequent hatching in zebrafish. In addition, there was early knockdown of expression of the pre-polster/anterior mesendoderm markers CatL, cap1, and BMP4. These results indicate zKLF4 is expressed within the pre-polster, an early mesendodermal site, and that it plays a critical role in the differentiation of these cells into hatching gland cells.
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