ZFIN ID: ZDB-PUB-050701-5
Structural and functional evidence for a singular repertoire of BMP receptor signal transducing proteins in the lophotrochozoan Crassostrea gigas suggests a shared ancestral BMP/activin pathway
Herpin, A., Lelong, C., Becker, T., Rosa, F., Favrel, P., and Cunningham, C.
Date: 2005
Source: The FEBS journal   272(13): 3424-3440 (Journal)
Registered Authors: Becker, Thomas S., Rosa, Frederic
Keywords: none
MeSH Terms:
  • Activin Receptors, Type I/genetics
  • Activin Receptors, Type I/metabolism
  • Activins/metabolism*
  • Amino Acid Sequence
  • Animals
  • Biological Evolution
  • Body Patterning
  • Bone Morphogenetic Protein Receptors, Type I
  • Bone Morphogenetic Proteins/metabolism*
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism
  • Gene Expression Regulation, Developmental*
  • Genes, Dominant
  • Larva/cytology
  • Larva/metabolism
  • Mesoderm/cytology
  • Mesoderm/metabolism
  • Molecular Sequence Data
  • Neoplasm Proteins/genetics
  • Neoplasm Proteins/metabolism
  • Ostreidae/embryology
  • Ostreidae/genetics*
  • Ostreidae/metabolism
  • Phenotype
  • Phylogeny
  • Protein-Serine-Threonine Kinases/genetics
  • Protein-Serine-Threonine Kinases/metabolism*
  • Receptors, Growth Factor/genetics
  • Receptors, Growth Factor/metabolism*
  • Receptors, Transforming Growth Factor beta/genetics
  • Receptors, Transforming Growth Factor beta/metabolism*
  • Signal Transduction*
  • Transforming Growth Factor beta/metabolism
  • Transforming Growth Factor beta/pharmacology
  • Zebrafish/embryology
  • Zebrafish/metabolism
PubMed: 15978047 Full text @ FEBS J.
The transforming growth factor beta (TGF-beta) superfamily includes bone morphogenetic proteins, activins and TGF-betasensu stricto (s.s). These ligands, which transduce their signal through a heteromeric complex of type I and type II receptors, have been shown to play a key role in numerous biological processes including early embryonic development in both deuterostomes and ecdyzozoans. Lophochotrozoans, the third major group of bilaterian animals, have remained in the background of the molecular survey of metazoan development. We report the cloning and functional study of the central part of the BMP pathway machinery in the bivalve mollusc Crassostrea gigas (Cg-BMPR1 type I receptor and Cg-TGFbetasfR2 type II receptor), showing an unusual functional mode of signal transduction for this superfamily. The use of the zebrafish embryo as a reporter organism revealed that Cg-BMPR1, Cg-TGFbetasfR2, Cg-ALR I, an activin Type I receptor or their dominant negative acting truncated forms, when overexpressed during gastrulation, resulted in a range of phenotypes displaying severe disturbance of anterioposterior patterning, due to strong modulations of ventrolateral mesoderm patterning. The results suggest that Cg-BMPR1, and to a certain degree Cg-TGFbetasfR2 proteins, function in C. gigas in a similar way to their zebrafish orthologues. Finally, based on phylogenetic analyses, we propose an evolutionary model within the complete TGF-beta superfamily. Thus, evidence provided by this study argues for a possible conserved endomesoderm/ectomesoderm inductive mechanism in spiralians through an ancestral BMP/activin pathway in which the singular, promiscuous and probably unique Cg-TGFbetasfR2 would be the shared type II receptor interface for both BMP and activin ligands.