PUBLICATION
Cre-mediated site-specific recombination in zebrafish embryos
- Authors
- Thummel, R., Burket, C.T., Brewer, J.L., Sarras, M.P. Jr., Li, L., Perry, M., McDermott, J.P., Sauer, B., Hyde, D.R., and Godwin, A.R.
- ID
- ZDB-PUB-050701-4
- Date
- 2005
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 233(4): 1366-1377 (Journal)
- Registered Authors
- Burket, Christopher, Thummel, Ryan
- Keywords
- conditional; Cre recombinase; hcRFP; heat shock; inducible; lacZ; transgenic; zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Embryo, Nonmammalian/physiology
- Genes, Reporter
- HSP70 Heat-Shock Proteins/genetics
- Integrases*
- Mosaicism
- Mutagenesis, Site-Directed*
- Recombinant Fusion Proteins/genetics
- Recombination, Genetic*
- Zebrafish/embryology*
- Zebrafish/genetics
- beta-Galactosidase/genetics
- PubMed
- 15977183 Full text @ Dev. Dyn.
Citation
Thummel, R., Burket, C.T., Brewer, J.L., Sarras, M.P. Jr., Li, L., Perry, M., McDermott, J.P., Sauer, B., Hyde, D.R., and Godwin, A.R. (2005) Cre-mediated site-specific recombination in zebrafish embryos. Developmental Dynamics : an official publication of the American Association of Anatomists. 233(4):1366-1377.
Abstract
Cre-mediated site-specific recombination has become an invaluable tool for manipulation of the murine genome. The ability to conditionally activate gene expression or to generate chromosomal alterations with this same tool would greatly enhance zebrafish genetics. This study demonstrates that the HSP70 promoter can be used to inducibly control expression of an enhanced green fluorescent protein (EGFP) -Cre fusion protein. The EGFP-Cre fusion protein is capable of promoting recombination between lox sites in injected plasmids or in stably inherited transgenes as early as 2 hr post-heat shock induction. Finally, the levels of Cre expression achieved in a transgenic fish line carrying the HSP70-EGFP-cre transgene are compatible with viability and both male and female transgenic fish are fertile subsequent to induction of EGFP-Cre expression. Hence, our data suggests that Cre-mediated recombination is a viable means of manipulating gene expression in zebrafish. Developmental Dynamics, 2005. (c) 2005 Wiley-Liss, Inc.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping