PUBLICATION

LZIC regulates neuronal survival during zebrafish development

Authors
Clements, W.K., and Kimelman, D.
ID
ZDB-PUB-050607-10
Date
2005
Source
Developmental Biology   283(2): 322-334 (Journal)
Registered Authors
Clements, Wilson, Kimelman, David
Keywords
LZIC; ICAT; ?-catenin; Zebrafish; Neural degeneration
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Brain/cytology
  • Brain/embryology*
  • Brain/metabolism
  • Cell Survival
  • Conserved Sequence
  • Cytoskeletal Proteins/metabolism
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism*
  • Humans
  • Intracellular Signaling Peptides and Proteins
  • Mice
  • Molecular Sequence Data
  • Neurons/physiology*
  • Oligonucleotides, Antisense/genetics
  • Protein Binding
  • Sequence Homology, Amino Acid
  • Trans-Activators/metabolism
  • Xenopus Proteins
  • Xenopus laevis/embryology
  • Xenopus laevis/metabolism
  • Zebrafish/embryology*
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • beta Catenin
PubMed
15932753 Full text @ Dev. Biol.
Abstract
Development of the brain and central nervous system is a complex process involving localized gene expression and regulated cell death and proliferation. Here, we describe a gene involved in neuronal survival, the zebrafish ortholog of the human lzic gene. Zebrafish lzic is expressed ubiquitously during early development and later becomes enriched in the developing brain. Using antisense morpholino oligonucleotides, we demonstrate that zebrafish lzic is required zygotically for the survival of distinct neuronal populations. LZIC is closely related to ICAT, a physiological inhibitor of the Wnt pathway that interacts physically and functionally with beta-catenin to prevent the transcription of Wnt target genes. LZIC's ICAT-homologous region is highly similar to ICAT with particular conservation of residues that are used by ICAT for beta-catenin-binding. Surprisingly, despite this high similarity, LZIC does not interact with beta-catenin in vitro or in vivo. Our results reveal that LZIC, a protein conserved in vertebrates, is required for neuronal survival in zebrafish.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping