PUBLICATION

Combinatorial activity of Flamingo proteins directs convergence and extension within the early zebrafish embryo via the planar cell polarity pathway

Authors
Formstone, C.J., and Mason, I.
ID
ZDB-PUB-050513-4
Date
2005
Source
Developmental Biology   282(2): 320-335 (Journal)
Registered Authors
Formstone, Caroline, Mason, Ivor
Keywords
Celsr; Flamingo; Gastrulation; Planar cell polarity; Cell movement; Embryo; Convergent extension; Wnt; Non-canonical pathway; Protocadherin; Neurulation
MeSH Terms
  • Animals
  • Base Sequence
  • Body Patterning/physiology*
  • Cadherins/genetics
  • Cadherins/metabolism*
  • Cell Polarity/physiology*
  • Cloning, Molecular
  • Cluster Analysis
  • DNA Primers
  • Glycoproteins/metabolism
  • Membrane Proteins/metabolism
  • Molecular Sequence Data
  • Phylogeny
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Signal Transduction/physiology*
  • Wnt Proteins
  • Zebrafish/embryology*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
15882862 Full text @ Dev. Biol.
Abstract
The seven-transmembrane protocadherin, Flamingo, functions in a number of processes during Drosophila development, including planar cell polarity (PCP). To assess the role(s) of Flamingo1/Celsr1 (Fmi1) during vertebrate embryogenesis we have exploited the zebrafish system, identifying two Fmi1 orthologues (zFmi1a and zFmi1b) and employing morpholinos to induce mis-splicing of zebrafish fmi1 mRNAs, to both imitate mutations identified in Drosophila flamingo and generate novel aberrant Flamingo proteins. We demonstrate that in the zebrafish gastrula, Fmi1 proteins function in concert with each other and with the vertebrate PCP proteins, Wnt11 and Strabismus, to mediate convergence and extension during gastrulation, without altering early dorso-ventral patterning. We show that zebrafish Fmi1a promotes extension of the entire antero-posterior axis of the zebrafish gastrula including prechordal plate and ventral diencephalic precursors. However, while we show that control over axial extension is autonomous, we find that Fmi1a is not required within lateral cells undergoing dorsal convergence.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes