PUBLICATION
Pathogenesis and inflammatory response to Edwardsiella tarda infection in the zebrafish
- Authors
- Pressley, M.E., Phelan, P.E. 3rd, Witten, P. Eckhard, Mellon, M.T., and Kim, C.H.
- ID
- ZDB-PUB-050309-2
- Date
- 2005
- Source
- Developmental and comparative immunology 29(6): 501-513 (Journal)
- Registered Authors
- Kim, Carol H., Witten, P. Eckhard
- Keywords
- Zebrafish; Pathogenesis; Edwardsiella tarda; Experimental infection; Inflammatory response
- MeSH Terms
-
- Animals
- Disease Models, Animal
- Edwardsiella tarda/immunology*
- Embryo, Nonmammalian/immunology
- Enterobacteriaceae Infections/immunology*
- Enterobacteriaceae Infections/pathology
- Female
- Histocytochemistry
- Inflammation/microbiology
- Interleukin-1/immunology
- RNA/chemistry
- RNA/genetics
- Reverse Transcriptase Polymerase Chain Reaction
- Tumor Necrosis Factor-alpha/immunology
- Zebrafish/genetics
- Zebrafish/immunology
- Zebrafish/microbiology*
- PubMed
- 15752547 Full text @ Dev. Comp. Immunol.
Citation
Pressley, M.E., Phelan, P.E. 3rd, Witten, P. Eckhard, Mellon, M.T., and Kim, C.H. (2005) Pathogenesis and inflammatory response to Edwardsiella tarda infection in the zebrafish. Developmental and comparative immunology. 29(6):501-513.
Abstract
The zebrafish (Danio rerio) is a widely used model for developmental biology, neurobiology, toxicology, and genetic disease. Recently, the zebrafish has been recognized as a valuable model for infectious disease and immunity. In this study the pathogenesis and inflammatory cytokine response of zebrafish to experimental Edwardsiella tarda infection was characterized. In challenge experiments, zebrafish embryos were susceptible to infection by immersion. Adult fish were susceptible to challenge by intraperitoneal (ip) injection but not static immersion unless the epithelial layer was perturbed by scraping prior to exposure. To determine if E. tarda infection induces a typical acute inflammatory response, mRNA expression levels of interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNFalpha) were assessed by quantitative real-time PCR. The expression levels of IL-1beta and TNFalpha were significantly upregulated in infected zebrafish embryos and adults. The methods developed in this study will be particularly valuable for targeted gene disruption studies of host immune components and in zebrafish genetic screens.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping