PUBLICATION
Two tyrosine hydroxylase genes in teleosts
- Authors
- Candy, J., and Collet, C.
- ID
- ZDB-PUB-050120-4
- Date
- 2005
- Source
- BBA Gene Structure and Expression 1727(1): 35-44 (Journal)
- Registered Authors
- Keywords
- Tyrosine hydroxylase; Teleost; Gene duplication
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Fishes/genetics*
- Molecular Sequence Data
- Phenylalanine Hydroxylase/genetics
- Phylogeny
- RNA, Messenger/metabolism
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Homology, Amino Acid
- Tyrosine 3-Monooxygenase/genetics*
- PubMed
- 15652156 Full text @ BBA Gene Structure and Expression
Citation
Candy, J., and Collet, C. (2005) Two tyrosine hydroxylase genes in teleosts. BBA Gene Structure and Expression. 1727(1):35-44.
Abstract
We report the finding of two non-allelic genes encoding tyrosine hydroxylase (TH) from the diploid teleost barramundi Lates calcarifer. Barramundi TH1 is the homologue of the higher vertebrate TH genes and encodes a protein of 489 amino acids that shares 90% sequence identity to the THs of other teleost species. A second non-allelic tyrosine hydroxylase gene (TH2) encodes a protein of 472 amino acids and shares 62% identity with TH1 and the vertebrate THs. TH1 mRNA is found in the brain and kidney of barramundi while TH2 mRNA is found only in brain. The TH2 gene is also present in the genomes of the pufferfish Takifugu and zebrafish Danio. Estimates of the rates of nucleotide substitution suggest the teleost TH2 genes are selectively constrained although not to the degree seen in the TH1 genes. Differential regulation of the two TH genes is, however, indicated by differences in transcript distribution, the nature of the Ca(2+)-responsive elements found in the proximal promoter region and the lack of recognised phosphorylation sites in TH2. Preservation of two apparently functional TH genes in phylogenetically distant teleost species is consistent with the notion of partitioning of function between duplicate genes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping