ZFIN ID: ZDB-PUB-041104-3
S100 protein-like immunoreactivity in the crypt olfactory neurons of the adult zebrafish
Germanà, A., Montalbano, G., Laurà, R., Ciriaco, E., Del Valle, M.E., and Vega, J.A.
Date: 2004
Source: Neuroscience letters 371(2-3): 196-198 (Journal)
Registered Authors: Germanà, Antonino, Montalbano, Giuseppe
Keywords: Crypt sensory neurons; Olfactory epithelium; S100 protein; Zebrafish
MeSH Terms:
  • Animals
  • Immunochemistry
  • Neurons/chemistry*
  • Neurons/metabolism
  • Olfactory Mucosa/chemistry*
  • Olfactory Mucosa/metabolism
  • S100 Proteins/analysis*
  • S100 Proteins/biosynthesis
  • Zebrafish/metabolism*
PubMed: 15519756 Full text @ Neurosci. Lett.
The olfactory epithelium of some teleosts, including zebrafish, contains three types of olfactory sensory neurons. Because zebrafish has become an ideal model for the study of neurogenesis in the olfactory system, it is of capital importance the identification of specific markers for different neuronal populations. In this study we used immunohistochemistry to analyze the distribution of S100 protein-like in the adult zebrafish olfactory epithelium. Surprisingly, specific S100 protein-like immunostaining was detected exclusively in crypt neurons, whereas ciliated and microvillous neurons were not reactive, and the supporting glial cells as well. The pattern of immunostaining was exclusively cytoplasmic without apparent polarity within the soma, and the intensity of immunostaining was not related with the maturative stage of the neurons. The role of S100 protein in crypt olfactory neurons is unknown, although it is probably associated with the capacity of these cells to respond to chemical stimuli. In any case, it represents an excellent marker to identify crypt olfactory neurons in zebrafish.