PUBLICATION
Ontogeny of the gut motility control system in zebrafish Danio rerio embryos and larvae
- Authors
- Holmberg, A., Schwerte, T., Pelster, B., and Holmgren, S.
- ID
- ZDB-PUB-041026-2
- Date
- 2004
- Source
- The Journal of experimental biology 207(Pt.23): 4085-4094 (Journal)
- Registered Authors
- Pelster, Bernd, Schwerte, Thorsten
- Keywords
- enteric nervous system, development, PACAP, tachykinin, acetylcholine, zebrafish, Danio rerio
- MeSH Terms
-
- Acetylcholine/pharmacology
- Animals
- Atropine/pharmacology
- Embryo, Nonmammalian/physiology
- Gastrointestinal Motility/drug effects*
- Gastrointestinal Motility/physiology
- Immunohistochemistry
- Larva/physiology
- Muscle, Smooth/drug effects*
- Muscle, Smooth/physiology
- Nerve Growth Factors/pharmacology
- Neurokinin A/pharmacology
- Neuropeptides/pharmacology
- Neurotransmitter Agents/pharmacology
- Pituitary Adenylate Cyclase-Activating Polypeptide
- Receptors, Vasoactive Intestinal Peptide
- Zebrafish/growth & development
- Zebrafish/physiology*
- Zebrafish Proteins
- PubMed
- 15498954 Full text @ J. Exp. Biol.
Citation
Holmberg, A., Schwerte, T., Pelster, B., and Holmgren, S. (2004) Ontogeny of the gut motility control system in zebrafish Danio rerio embryos and larvae. The Journal of experimental biology. 207(Pt.23):4085-4094.
Abstract
Using digital motion analysis, the ontogeny of the cholinergic, tachykinin and pituitary adenylate cyclase-activating polypeptide (PACAP) control systems was studied in zebrafish Danio rerio larvae, in vivo. For the first time we show that the regular propagating anterograde waves that occur in the zebrafish larval gut before and around the onset [at 5-6 days post fertilization (d.p.f.)] of feeding are modulated by acetylcholine or atropine, PACAP and NKA (neurokinin A). At 3 d.p.f., when no spontaneous motility has developed, application of acetylcholine did not affect the gut. However, at 4 d.p.f., acetylcholine increased and atropine reduced the frequency of propagating anterograde waves. At 5 d.p.f., NKA increased and PACAP reduced the wave frequency. This suggests that both excitatory and inhibitory pathways develop at an early stage in the gut, independent of exogenous feeding. Immunohistochemistry established the presence of gut neurons expressing PACAP and NKA in the proximal part of the developing gut from the first stage investigated (2 d.p.f.) and before regular motility was observed. 1 d.p.f. (PACAP) or 2 d.p.f. (NKA) stages later the whole gut was innervated. This supports physiological results that gut motility is under neuronal control during the period when regular motility patterns develop.
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