PUBLICATION
Separable enhancer sequences regulate the expression of the neural bHLH transcription factor neurogenin 1
- Authors
- Nakada, Y., Parab, P., Simmons, A., Omer-Abdalla, A., and Johnson, J.E.
- ID
- ZDB-PUB-040630-2
- Date
- 2004
- Source
- Developmental Biology 271(2): 479-487 (Journal)
- Registered Authors
- Keywords
- Transcriptional regulation, Transgenic mice, Dorsal–neural tube, Ventral–neural tube, Basic helix-loop-helix transcription factor, Neurogenin 1, Spinal cord development
- MeSH Terms
-
- Animals
- Base Sequence
- Basic Helix-Loop-Helix Transcription Factors
- Central Nervous System/metabolism*
- DNA Primers
- Enhancer Elements, Genetic/genetics*
- Gene Components
- Gene Expression Regulation, Developmental*
- Helix-Loop-Helix Motifs/genetics*
- In Situ Hybridization
- Lac Operon/genetics
- Mice
- Mice, Transgenic
- Molecular Sequence Data
- Nerve Tissue Proteins/genetics
- Nerve Tissue Proteins/metabolism*
- Transcription Factors/genetics
- Transcription Factors/metabolism*
- Transgenes/genetics
- Zebrafish Proteins*
- PubMed
- 15223348 Full text @ Dev. Biol.
Citation
Nakada, Y., Parab, P., Simmons, A., Omer-Abdalla, A., and Johnson, J.E. (2004) Separable enhancer sequences regulate the expression of the neural bHLH transcription factor neurogenin 1. Developmental Biology. 271(2):479-487.
Abstract
Ngn1 is a basic helix-loop-helix (bHLH) transcription factor expressed in specific regions within the developing brain and spinal cord, sensory ganglia, and olfactory epithelium. We have identified sequences both 5' and 3' of the mouse ngn1 gene that function in regulating ngn1 expression, and each of these sequences contains distinct regulatory cassettes for different subregions of the expression domain. Enhancers for expression in ngn1 domains of the midbrain, hindbrain, trigeminal ganglia, and ventral-neural tube appear redundant and are spread both 5' and 3' of the ngn1 coding sequence. In contrast, a single discrete dorsal-neural tube enhancer was located in the 5' sequence that is conserved among mouse, human, chick, and zebrafish ngn1 genes. Functionally, this enhancer is both necessary and sufficient for driving expression of a heterologous reporter in transgenic mice specifically to the dorsal domain of ngn1 expression in the spinal neural tube. Thus, sequences are identified that can be used to direct temporally and spatially restricted expression of heterologous genes to the developing neural tube.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping