PUBLICATION
Transcriptional and translational regulation of zebrafish connexin 55.5 (zf.Cx.55.5) and connexin 52.6 (zf.Cx52.6)
- Authors
- Hussain, M.U., Kremer, M., Zoidl, G., and Dermietzel, R.
- ID
- ZDB-PUB-040113-5
- Date
- 2003
- Source
- Cell communication & adhesion 10(4-6): 227-231 (Review)
- Registered Authors
- Zoidl, Georg
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Communication/genetics
- Cell Communication/physiology*
- Cells, Cultured
- Connexins/genetics
- Connexins/metabolism*
- Genes, Reporter/genetics
- Green Fluorescent Proteins
- HeLa Cells
- Humans
- Luminescent Proteins/genetics
- Luminescent Proteins/metabolism
- Promoter Regions, Genetic/genetics*
- Recombinant Fusion Proteins/genetics
- Recombinant Fusion Proteins/metabolism*
- Zebrafish/genetics
- PubMed
- 14681021
Citation
Hussain, M.U., Kremer, M., Zoidl, G., and Dermietzel, R. (2003) Transcriptional and translational regulation of zebrafish connexin 55.5 (zf.Cx.55.5) and connexin 52.6 (zf.Cx52.6). Cell communication & adhesion. 10(4-6):227-231.
Abstract
Zebrafish connexin 55.5 (zf.Cx55.5) and connexin 52.6 (zf.Cx52.6) show highly restricted expression patterns in the nervous system. Both connexins are confined to subsets of neurons in the fish retina. In order to get initial answers to the questions of pattern definition in neuronal subsets, we elucidated molecular mechanisms responsible for their expression. Different upstream DNA fragments were subcloned into a pGL3-basic vector and transiently transfected in HeLa and N2A cells. Luciferase activity showed the presence of two putative promoter elements in zfCx55.5 and a promoter element in zfCx52.6 that showed different promoter activities in HeLa and N2A cells. Moreover, fusion constructs of zfCx55.5 with EGFP revealed the presence of a new isoform with an additional short exon I.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping