Zebrafish sperm cryopreservation with N,N-dimethylacetamide
- Morris IV, J.P., Berghmans, S., Zahrieh, D., Neuberg, D.S., Kanki, J.P., and Look, A.T.
- Biotechniques 35(5): 956-958 (Journal)
- Registered Authors
- Kanki, John, Look, A. Thomas
- MeSH Terms
- Cell Membrane Permeability/drug effects
- Cryoprotective Agents/pharmacology*
- Fertility/drug effects
- Semen Preservation/methods*
- Sperm Motility/drug effects*
- Spermatozoa/drug effects*
- 14628669 Full text @ Biotechniques
Morris IV, J.P., Berghmans, S., Zahrieh, D., Neuberg, D.S., Kanki, J.P., and Look, A.T. (2003) Zebrafish sperm cryopreservation with N,N-dimethylacetamide. Biotechniques. 35(5):956-958.
High fecundity, rapid generation time, and external development of optically clear embryos make the zebrafish (Danio rerio) a convenient vertebrate model for genetic, developmental, and disease studies. Efficient sperm cryopreservation enhances the zebrafish model system by optimizing productive use of facility space, extending the reproductive lifetime of males, providing an alternative to live stocks for strain recovery, and ensuring the survival of valuable mutant lines. Here we identify a cryoprotective medium, 10% N,N-dimethylacetamide (DMA) (v/v) diluted in buffered sperm motility-inhibiting solution (BSMIS), as well as parameters for zebrafish sperm cryopreservation that enhance cryopreservation efficiency and significantly increase the yield of live embryos from archived stocks. Our experiments emphasize the effect of the ratio of sperm and medium volume and the use of large egg clutches to maximize the recovery of viable embryos.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes