PUBLICATION

Analysis of zebrafish cyp19 promoters

Authors
Tong, S.K. and Chung, B.
ID
ZDB-PUB-031119-1
Date
2003
Source
Journal of steroid biochemistry   86(3-5): 381-386 (Journal)
Registered Authors
Chung, Bon-chu
Keywords
Ovary; Brain; Promoter; Aromatase; cyp19; P450aro; Zebrafish
MeSH Terms
  • Animals
  • Aromatase/genetics*
  • Aromatase/metabolism
  • Base Sequence
  • Binding Sites
  • DNA-Binding Proteins
  • Humans
  • Molecular Sequence Data
  • Promoter Regions, Genetic/genetics
  • Receptors, Cytoplasmic and Nuclear
  • Steroidogenic Factor 1
  • Tissue Distribution
  • Transcription Factors/metabolism
  • Zebrafish/genetics*
  • Zebrafish/metabolism*
  • Zebrafish Proteins
PubMed
14623534 Full text @ J. Steroid Biochem.
Abstract
Cyp19 encodes P450 aromatase, the key enzyme catalyzing the conversion of androgens into estrogens. Estrogens play a crucial role in the anatomical, functional and behavioral characteristics of sexually dimorphic development. In zebrafish, two cyp19 genes, cyp19a and cyp19b, expressed in ovary and brain, respectively, were found. We have isolated the promoter regions of the zebrafish cyp19 genes from a bacterial artificial chromosome library to search for regulatory sequences that bind to transcription factors. Sequences like arylhydrocarbon receptor (AhR) recognition site, estrogen receptor recognition half sites (1/2ERE) and c-AMP responsive elements were found in the 5'-flanking regions of both cyp19 genes. For ovarian-specific expression, we found binding sites for steroidogenic factor-1 (SF-1), GATA transcription factor 4 (GATA-4) and Wilm tumor 1 (WT1-KTS) on the promoter region of cyp19a but not cyp19b. For brain-specific expression of the cyp19b gene, sequences for recognition of chicken ovalbumin upstream promoter-transcription factor (COUP) and Ptx-1 were detected in the promoter. The importance of these putative control elements in ovary and brain-specific promoter has been assessed by sequence comparison among various species.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping