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ZIRC
ZFIN ID: ZDB-PUB-030826-2
Transposition and targeting of the prokaryotic mobile element IS30 in zebrafish
Szabo, M., Müller, F., Kiss, J., Balduf, C., Strähle, U., and Olasz, F.
Date: 2003
Source: FEBS letters   550(1-3): 46-50 (Journal)
Registered Authors: Balduf, Carolin, Müller, Ferenc, Strähle, Uwe, Szabo, Marianne
Keywords: none
MeSH Terms:
  • Animals
  • Binding Sites
  • DNA/metabolism
  • DNA Transposable Elements*
  • DNA-Binding Proteins*
  • Embryo, Nonmammalian
  • Escherichia coli/genetics
  • Genetic Techniques
  • HeLa Cells
  • Humans
  • Oncogene Proteins/genetics
  • Oncogene Proteins/metabolism
  • Prokaryotic Cells/physiology
  • Recombination, Genetic
  • Repressor Proteins/genetics
  • Repressor Proteins/metabolism
  • Substrate Specificity
  • Trans-Activators
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • Transposases/genetics
  • Transposases/metabolism
  • Viral Proteins
  • Viral Regulatory and Accessory Proteins
  • Zebrafish/embryology
  • Zebrafish/genetics*
PubMed: 12935884 Full text @ FEBS Lett.
ABSTRACT
We provide evidence that a prokaryotic insertion sequence (IS) element is active in a vertebrate system. The transposase of Escherichia coli element IS30 catalyzes both excision and integration in extrachromosomal DNA in zebrafish embryos. The transposase has a pronounced target preference, which is shown to be modified by fusing the enzyme to unrelated DNA binding proteins. Joining the transposase to the cI repressor of phage lambda causes transposition primarily into the vicinity of the lambda operator in E. coli, and linking to the DNA binding domain of Gli1 also directs the recombination activity of transposase near to the Gli1 binding site in zebrafish. Our results demonstrate the possibility of fusion transposases to acquire novel target specificity in both prokaryotes and eukaryotes.
ADDITIONAL INFORMATION No data available