PUBLICATION

Heparan sulfate 6-O-sulfotransferase is essential for muscle development in zebrafish

Authors
Bink, R.J., Habuchi, H., Lele, Z., Dolk, E., Joore, J., Rauch, G.J., Geisler, R., Wilson, S.W., den Hertog, J., Kimata, K., and Zivkovic, D.
ID
ZDB-PUB-030716-29
Date
2003
Source
The Journal of biological chemistry   278(33): 31118-31127 (Journal)
Registered Authors
den Hertog, Jeroen, Geisler, Robert, Jongejan-Zivkovic, Dana, Joore, Jos, Lele, Zsolt, Rauch, Gerd-Jörg, Wilson, Steve
Keywords
none
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Cell Differentiation
  • Gene Expression Regulation, Developmental
  • Gene Expression Regulation, Enzymologic
  • Heparan Sulfate Proteoglycans/metabolism*
  • Molecular Sequence Data
  • Muscle, Skeletal/cytology
  • Muscle, Skeletal/embryology*
  • Muscle, Skeletal/enzymology*
  • Oligonucleotides, Antisense/pharmacology
  • Phenotype
  • Somites/enzymology
  • Sulfotransferases/genetics*
  • Sulfotransferases/metabolism*
  • Zebrafish/genetics*
PubMed
12782624 Full text @ J. Biol. Chem.
Abstract
Heparan sulfate proteoglycans function in development and disease. They consist of a core protein with attached heparan sulfate chains that are altered by a series of carbohydrate-modifying enzymes and sulfotransferases. Here, we report on the identification and characterization of a gene encoding zebrafish heparan sulfate 6-O-sulfotransferase that shows high homology to other heparansulfate 6-O-sulfotransferases. When expressed as a fusion protein in cultured cells, the protein shows specific 6-O-sulfotransferase activity and preferentially acts on the iduronosyl N-sulfoglycosamine. In the developing embryo, hs6st is expressed in the brain, the somites and the fins; the same structures that were affected upon morpholino-mediated functional knockdown. Morpholino injections significantly inhibited 6-O- but not 2-O-sulfation as assessed by HPLC. Morphants display disturbed somite specification independent of the somite oscillator mechanism and have impaired muscle differentiation. In conclusion, our results show that transfer of sulfate to specific positions on glycosaminglycans is essential for muscle development.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes