ZFIN ID: ZDB-PUB-030408-8
Teleost multiple tissue (tmt) opsin: a candidate photopigment regulating the peripheral clocks of zebrafish?
Moutsaki, P., Whitmore, D., Bellingham, J., Sakamoto, K., David-Gray, Z.K., and Foster, R.G.
Date: 2003
Source: Brain research. Molecular brain research   112(1-2): 135-145 (Journal)
Registered Authors: Whitmore, David
Keywords: none
MeSH Terms:
  • Amino Acid Sequence/genetics
  • Animals
  • Base Sequence/genetics
  • Biological Clocks/physiology*
  • Brain/metabolism
  • Cell Line/cytology
  • Cell Line/metabolism*
  • DNA, Complementary/analysis
  • DNA, Complementary/genetics
  • Evolution, Molecular
  • Gene Expression Regulation/genetics*
  • Genomic Library
  • Molecular Sequence Data
  • Photoperiod*
  • Phylogeny
  • Rod Opsins/genetics
  • Rod Opsins/isolation & purification*
  • Sequence Homology, Amino Acid
  • Takifugu/genetics
  • Takifugu/metabolism*
  • Viscera/metabolism
  • Zebrafish/genetics
  • Zebrafish/metabolism*
PubMed: 12670711 Full text @ Brain Res. Mol. Brain Res.
ABSTRACT
Isolated organs and cell lines from zebrafish exhibit circadian oscillations in clock gene expression that can be entrained to a 24-h light/dark cycle. The mechanism underlying this cellular photosensitivity is unknown. We report the identification of a novel opsin family, tmt-opsin, that has a genomic structure characteristic of vertebrate photopigments, an amino acid identity equivalent to the known photopigment opsins, and the essential residues required for photopigment function. Significantly, tmt-opsin is expressed in a wide variety of neural and non-neural tissues, including a zebrafish embryonic cell line that exhibits a light entrainable clock. Collectively the data suggest that tmt-opsin is a strong candidate for the photic regulation of zebrafish peripheral clocks.
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