ZFIN ID: ZDB-PUB-030408-5
The role of Ppt/Wnt5 in regulating cell shape and movement during zebrafish gastrulation
Kilian, B., Mansukoski, H., Barbosa, F.C., Ulrich, F., Tada, M., and Heisenberg, C.P.
Date: 2003
Source: Mechanisms of Development   120(4): 467-476 (Journal)
Registered Authors: Heisenberg, Carl-Philipp, Kilian, Beate, Mansukoski, Hannu, Tada, Masazumi, Ulrich, Florian
Keywords: none
MeSH Terms:
  • Animals
  • Cell Lineage
  • Gastrula/metabolism
  • Gastrula/physiology*
  • Gene Expression Regulation, Developmental*
  • Glycoproteins/genetics
  • Glycoproteins/physiology
  • In Situ Hybridization
  • Microscopy, Confocal
  • Mutation
  • Oligonucleotides, Antisense/pharmacology
  • Phenotype
  • Proto-Oncogene Proteins/physiology*
  • Receptors, Neurotransmitter/metabolism
  • Signal Transduction
  • Time Factors
  • Transcription Factors/genetics
  • Transcription Factors/physiology*
  • Wnt Proteins
  • Zebrafish
  • Zebrafish Proteins/physiology*
PubMed: 12676324 Full text @ Mech. Dev.
Wnt genes play important roles in regulating patterning and morphogenesis during vertebrate gastrulation. In zebrafish, slb/wnt11 is required for convergence and extension movements, but not cell fate specification during gastrulation. To determine if other Wnt genes functionally interact with slb/wnt11, we analysed the role of ppt/wnt5 during zebrafish gastrulation. ppt/wnt5 is maternally provided and zygotically expressed at all stages during gastrulation. The analysis of ppt mutant embryos reveals that Ppt/Wnt5 regulates cell elongation and convergent extension movements in posterior regions of the gastrula, while its function in more anterior regions is largely redundant to that of Slb/Wnt11. Frizzled-2 functions downstream of ppt/wnt5, indicating that it might act as a receptor for Ppt/Wnt5 in this process. The characterisation of the role of Ppt/Wnt5 provides insight into the functional diversity of Wnt genes in regulating vertebrate gastrulation movements.