PUBLICATION
Multiple functions of LIM domain-binding CLIM/NLI/Ldb cofactors during zebrafish development
- Authors
- Becker, T., Ostendorff, H., Bossenz, M., Schlüter, A., Becker, C.G., Peirano, R.I., and Bach, I.
- ID
- ZDB-PUB-020912-7
- Date
- 2002
- Source
- Mechanisms of Development 117(1-2): 75-85 (Journal)
- Registered Authors
- Becker, Catherina G., Becker, Thomas
- Keywords
- LIM homeodomain factor; CLIM/NLI/Ldb cofactor; eye; midbrain-hindbrain boundary; axonal projection; zebrafish
- MeSH Terms
-
- Animals
- Axons/metabolism
- Axons/ultrastructure
- Brain/embryology
- Brain/metabolism
- Eye/embryology
- Eye/metabolism
- Gene Expression Regulation, Developmental
- Genes, Homeobox
- Homeodomain Proteins/genetics
- Homeodomain Proteins/metabolism*
- Immunohistochemistry
- In Situ Hybridization
- Neurons/metabolism
- Transcription Factors/genetics
- Transcription Factors/metabolism*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism*
- PubMed
- 12204249 Full text @ Mech. Dev.
Citation
Becker, T., Ostendorff, H., Bossenz, M., Schlüter, A., Becker, C.G., Peirano, R.I., and Bach, I. (2002) Multiple functions of LIM domain-binding CLIM/NLI/Ldb cofactors during zebrafish development. Mechanisms of Development. 117(1-2):75-85.
Abstract
The crucial involvement of CLIM/NLI/Ldb cofactors for the exertion of the biological activity of LIM homeodomain transcription factors (LIM-HD ) has been demonstrated. In this paper we show that CLIM cofactors are widely expressed during zebrafish development with high protein levels in specific neuronal cell types where LIM-HD proteins of the Isl class are synthesized. The overexpression of a dominant-negative CLIM molecule (DN-CLIM) that contains the LIM interaction domain (LID) during early developmental stages of zebrafish embryos results in an impairment of eye and midbrain-hindbrain boundary (MHB) development and disturbances in the formation of the anterior midline. On a cellular level we show that the outgrowth of peripheral but not central axons from Rohon Beard (RB) and trigeminal sensory neurons is inhibited by DN-CLIM overexpression. We demonstrate a further critical role of CLIM cofactors for axonal outgrowth of motor neurons. Additionally, DN-CLIM overexpression causes an increase of Isl-protein expression levels in specific neuronal cell types, likely due to a protection of the DN-CLIM/ LIM-HD complex from proteasomal degradation. Our results demonstrate multiple roles of the CLIM cofactor family for the development of entire organs, axonal outgrowth of specific neurons and protein expression levels.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping