PUBLICATION
Analysis of xanthophore and pterinosome biogenesis in zebrafish using methylene blue and pteridine autofluorescence
- Authors
- Le Guyader, S. and Jesuthasan, S.
- ID
- ZDB-PUB-020218-5
- Date
- 2002
- Source
- Pigment cell research 15(1): 27-31 (Journal)
- Registered Authors
- Jesuthasan, Suresh, Le Guyader, Sylvie
- Keywords
- none
- MeSH Terms
-
- Animals
- Fluorescence
- Light
- Methylene Blue/metabolism
- Pteridines/metabolism*
- Pterins*
- Ultraviolet Rays
- Zebrafish/embryology*
- PubMed
- 11837453 Full text @ Pig. Cell Res.
Citation
Le Guyader, S. and Jesuthasan, S. (2002) Analysis of xanthophore and pterinosome biogenesis in zebrafish using methylene blue and pteridine autofluorescence. Pigment cell research. 15(1):27-31.
Abstract
We have identified two simple methods to analyse xanthophore and pterinosome biogenesis in zebrafish. The first uses methylene blue (methylthionium chloride), a redox dye which specifically labels xanthophores and pterinosomes, while the second uses autofluorescence to detect pteridine levels; these methods may be used to detect the number, location and shape of xanthophores and pterinosomes. These assays were applied to two zebrafish mutants--brie and yobo--and revealed that both mutants have pterinosome biogenesis and pteridine synthesis defects. Additionally, using capillary electrophoresis, we provide evidence that sepiapterin is responsible for the yellow colour and blue-light induced fluorescence in zebrafish embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping