ZFIN ID: ZDB-PUB-010601-13
Heat shock stimulation of a tilapia heat shock protein 70 promoter is mediated by a distal element
Molina, A., Di Martino, E., Martial, J.A., and Muller, M.
Date: 2001
Source: The Biochemical journal 356(2): 353-359 (Journal)
Registered Authors: Martial, Joseph A., Molina, Alfredo, Muller, Marc
Keywords: HSP70; transcription; zebrafish
MeSH Terms:
  • Animals
  • Base Sequence
  • Binding Sites/genetics
  • Carps/genetics
  • Cells, Cultured
  • DNA/genetics
  • DNA/metabolism
  • DNA Primers/genetics
  • Gene Expression
  • Genes, Reporter
  • HSP70 Heat-Shock Proteins/genetics*
  • Hot Temperature
  • Mutagenesis, Site-Directed
  • Promoter Regions, Genetic*
  • Sequence Homology, Amino Acid
  • Tilapia/genetics*
  • Transcription Factors/metabolism
  • Zebrafish/embryology
  • Zebrafish/genetics
PubMed: 11368761 Full text @ Biochem. J.
We reported previously that a tilapia (Oreochromis mossambicus) heat shock protein 70 (HSP70) promoter is able to confer heat shock response on a reporter gene after transient expression both in cell culture and in microinjected zebrafish embryos. Here we present the first functional analysis of a fish HSP70 promoter, the tiHSP70 promoter. Using transient expression experiments in carp EPC (epithelioma papulosum cyprini) cells and in microinjected zebrafish embryos, we show that a distal heat shock response element (HSE1) at approx. -800 is predominantly responsible for the heat shock response of the tiHSP70 promoter. This element specifically binds an inducible transcription factor, most probably heat shock factor, and a constitutive factor. The constitutive complex is not observed with the non-functional, proximal HSE3 sequence, suggesting that both factors are required for the heat shock response mediated by HSE1.