PUBLICATION

Two-color detection of mRNA transcript localizations in fish and fly embryos using alkaline phosphatase and beta-galactosidase conjugated antibodies

Authors
Hauptmann, G.
ID
ZDB-PUB-010404-13
Date
1999
Source
Development genes and evolution   209(5): 317-321 (Journal)
Registered Authors
Hauptmann, Giselbert
Keywords
none
MeSH Terms
  • Alkaline Phosphatase/immunology*
  • Animals
  • Drosophila/embryology*
  • Embryo, Nonmammalian/metabolism
  • Fishes/embryology*
  • Immunohistochemistry
  • Nucleic Acid Hybridization
  • RNA, Messenger/genetics*
  • beta-Galactosidase/immunology*
PubMed
11252185 Full text @ Dev. Genes Evol.
Abstract
A fast and simplified two-color in situ hybridization procedure for the simultaneous detection of two different mRNAs in whole-mounted zebrafish and Drosophila embryos is presented. Transcript distributions are detected in a single incubation step using a mixture of alkaline phosphatase and beta-galactosidase coupled antibodies. The different transcripts are visualized in contrasting colors by the use of beta-galactosidase substrates that develop color precipitates (magenta, blue) easily distinguishable from those of the standard alkaline phosphatase substrates. This protocol can be followed by standard immunohistochemistry to detect the expression of a third gene (at the protein level) in a third color.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping