PUBLICATION
Nitric oxide and vascular reactivity in developing zebrafish, Danio rerio
- Authors
- Fritsche, R., Schwerte, T., and Pelster, B.
- ID
- ZDB-PUB-001205-11
- Date
- 2000
- Source
- American journal of physiology. Regulatory, integrative and comparative physiology 279(6): R2200-R2207 (Journal)
- Registered Authors
- Pelster, Bernd, Schwerte, Thorsten
- Keywords
- ontogeny; nitric oxide synthase; epinephrine; cardiac; immunohistochemistry
- MeSH Terms
-
- Animals
- Arteries/drug effects
- Arteries/physiology*
- Cardiac Output
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/physiology
- Endothelium, Vascular/drug effects
- Endothelium, Vascular/physiology
- Epinephrine/pharmacology
- Erythrocyte Count
- Larva
- Models, Cardiovascular
- Muscle, Smooth, Vascular/drug effects
- Muscle, Smooth, Vascular/physiology*
- NG-Nitroarginine Methyl Ester/pharmacology
- Nitric Oxide/pharmacology
- Nitric Oxide/physiology*
- Nitroprusside/pharmacology
- Vascular Resistance/drug effects
- Vascular Resistance/physiology
- Vasoconstriction/drug effects
- Vasodilation/drug effects
- Veins/drug effects
- Veins/physiology*
- Video Recording
- Zebrafish/physiology
- PubMed
- 11080086 Full text @ Am. J. Physiol. Regul. Integr. Comp. Physiol.
Citation
Fritsche, R., Schwerte, T., and Pelster, B. (2000) Nitric oxide and vascular reactivity in developing zebrafish, Danio rerio. American journal of physiology. Regulatory, integrative and comparative physiology. 279(6):R2200-R2207.
Abstract
We used a newly developed digital motion analysis video technique to study the effects of nitric oxide (NO) and epinephrine on the early larval arterial and venous vasculature of zebrafish. Application of the NO donor sodium nitroprusside resulted in a significant increase in both the venous and arterial vessel diameters, whereas N(G)-nitro-L-arginine methyl ester caused a significant decrease in the same diameters. Thus our results show that both the venous and arterial vasculature of the 5- and 6-day-old zebrafish larvae are influenced by endogenously produced NO. By use of immunohistochemistry, NO synthase immunoreactivity was demonstrated in endothelial cells of the dorsal vein. Local application of epinephrine onto the dorsal artery had no effect on vessel diameter. However, if the embryos were preincubated with N(omega)-nitro-L-arginine methyl ester, addition of epinephrine resulted in a significant reduction in both arterial and venous vessel diameters. Thus this study provides increasing evidence that before a functional autonomic innervation of the peripheral vascular system, vascular tone in larval tissue is regulated by a complex interaction of vasoactive substances that are produced locally by vascular endothelial cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping