PUBLICATION
Identification of a histamine H3-like receptor in the zebrafish (Danio rerio) brain
- Authors
- Peitsaro, N., Anichtchik, O.V., and Panula, P.
- ID
- ZDB-PUB-000810-2
- Date
- 2000
- Source
- Journal of neurochemistry 75(2): 718-724 (Journal)
- Registered Authors
- Anichtchik, Oleg, Panula, Pertti, Peitsaro, Nina
- Keywords
- histamine; zebrafish; H-3 receptor; guanosine 5'-(gamma-[S-35]thio); triphosphate; autoradiography; Danio rerio
- MeSH Terms
-
- Animals
- Autoradiography
- Brain/cytology
- Brain/metabolism*
- Cimetidine/pharmacology
- Female
- Guanosine 5'-O-(3-Thiotriphosphate)/pharmacokinetics
- Histamine Agonists/pharmacokinetics
- Histamine Antagonists/pharmacology
- Imidazoles/pharmacology
- Kinetics
- Male
- Methylhistamines/pharmacokinetics
- Piperidines/pharmacology
- Pyrilamine/pharmacology
- Radioligand Assay
- Receptors, Histamine H3/analysis
- Receptors, Histamine H3/metabolism*
- Sulfur Radioisotopes
- Thiourea/analogs & derivatives
- Thiourea/pharmacology
- Tritium
- Zebrafish
- PubMed
- 10899947 Full text @ J. Neurochem.
- CTD
- 10899947
Citation
Peitsaro, N., Anichtchik, O.V., and Panula, P. (2000) Identification of a histamine H3-like receptor in the zebrafish (Danio rerio) brain. Journal of neurochemistry. 75(2):718-724.
Abstract
The distribution of histaminergic fibers in the zebrafish brain was recently shown to resemble that in mammals. Expression of L-histidine decarboxylase (HDC) mRNA was shown only in the area corresponding to that expressing HDC in mammals. This indicates that the zebrafish could be a useful model for studies on the function of the brain histaminergic system. In this study an H(3)-like receptor is identified in zebrafish brain. With binding studies using N-alpha-[(3)H]methylhistamine on zebrafish brain sections, signals were observed in several regions. Highest densities were detected in optic tectum and hypothalamus. The autoradiographic signal was abolished completely by the H(3)-specific antagonist clobenpropit and significantly reduced by another H(3) antagonist, thioperamide. Histamine and immepip induced an increase of guanosine 5'-(gamma-[(35)S]thio)triphosphate binding in several areas of the zebrafish brain. The activation was blocked with clobenpropit but not with cimetidine or mepyramine. These results indicate that the zebrafish has a histamine H(3)-like receptor that functionally interacts with the inhibitory, G(i)/G(o), class of G proteins. No previous evidence for a histamine receptor in zebrafish exists. The receptor described here is apparently similar to the mammalian H(3) receptor, making this the first description of a histamine H(3)-like receptor in a lower vertebrate.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping