PUBLICATION
Identification of seven genes in the major histocompatibility complex class I region of the zebrafish
- Authors
- Sültmann, H., Murray, B.W., and Klein, J.
- ID
- ZDB-PUB-000623-1
- Date
- 2000
- Source
- Scandinavian journal of immunology 51(6): 577-585 (Journal)
- Registered Authors
- Klein, Jan, Sültmann, Holger
- Keywords
- none
- MeSH Terms
-
- ATP-Binding Cassette Transporters/genetics
- ATP-Binding Cassette Transporters/isolation & purification
- ATP-Binding Cassette Transporters/metabolism
- Adaptor Proteins, Signal Transducing
- Amino Acid Sequence
- Animals
- Antiporters/genetics
- Antiporters/isolation & purification
- Base Sequence
- Carrier Proteins/genetics
- Carrier Proteins/isolation & purification
- DNA-Binding Proteins/genetics
- DNA-Binding Proteins/isolation & purification
- Genes, MHC Class I/genetics*
- Humans
- Immunoglobulins/genetics
- Immunoglobulins/isolation & purification
- Intracellular Signaling Peptides and Proteins*
- Kinesins/chemistry
- Kinesins/genetics
- Kinesins/isolation & purification
- Membrane Transport Proteins
- Mice
- Molecular Sequence Data
- Nuclear Proteins*
- Oxidoreductases*
- Proteins/genetics
- Proteins/isolation & purification
- Zebrafish/genetics*
- Zebrafish/immunology*
- Zebrafish Proteins*
- PubMed
- 10849368 Full text @ Scand. J. Immunol.
Citation
Sültmann, H., Murray, B.W., and Klein, J. (2000) Identification of seven genes in the major histocompatibility complex class I region of the zebrafish. Scandinavian journal of immunology. 51(6):577-585.
Abstract
Physical linkage of genes whose products are involved in similar physiological pathways may have functional significance. The identification of conserved gene linkage in distantly related organisms can therefore strengthen the hypothesis of selection acting towards keeping genes on a chromosome. We used the cDNA selection technique and the polymerase chain reaction (PCR) with generic primers for the identification of new genes on the genomic clones bearing the major histocompatibility complex (Mhc) class I genes of the zebrafish (Danio rerio). We found six new genes (BING1, DAXX, TAPBP, KNSL2, TAP2B and KE6) whose orthologues are known to be linked to the Mhc class II region in humans and mice. In addition, a new zebrafish Mhc class I gene, termed Dare-UFA, was detected. By contrast, a search for the human leucocyte antigen (HLA)-linked BING3, KE3 and SACM2L genes revealed that these loci are not located on the class I clones of the zebrafish. The zebrafish class I region contains repetitive elements with similarity to the DANA, SATA and LINE repeats, as well as Tc1 transposable elements. Our findings indicate a high degree of linkage conservation between the zebrafish class I and the mammalian class II regions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping