PUBLICATION
Cell turnover in neuromasts of zebrafish larvae
- Authors
- Williams, J.A. and Holder, N.
- ID
- ZDB-PUB-000505-12
- Date
- 2000
- Source
- Hearing Research 143(1-2): 171-81 (Journal)
- Registered Authors
- Holder, Nigel, Williams, Juliet
- Keywords
- zebrafish; neuromast; cell turnover
- MeSH Terms
-
- Amino Acid Chloromethyl Ketones/pharmacology
- Animals
- Apoptosis
- Cell Death/drug effects
- Cell Death/physiology
- Cell Differentiation/physiology
- Cell Division/physiology
- Cell Movement
- Cysteine Proteinase Inhibitors/pharmacology
- Hair Cells, Auditory/cytology
- Larva/cytology
- Larva/physiology
- Larva/ultrastructure
- Neomycin/pharmacology
- Sense Organs/cytology*
- Sense Organs/physiology
- Sense Organs/ultrastructure
- Zebrafish/growth & development*
- PubMed
- 10771194 Full text @ Hear. Res.
Citation
Williams, J.A. and Holder, N. (2000) Cell turnover in neuromasts of zebrafish larvae. Hearing Research. 143(1-2):171-81.
Abstract
The numbers and positions of cells undergoing cell death and proliferation in the neuromasts of 10 day old zebrafish larvae were assessed to investigate the ability of supporting cells to differentiate into hair cells. Evaluations of cell death and proliferation showed that a subpopulation of cells located in the centre of the neuromast undergo cell death, and a different subpopulation located at the periphery proliferate. This suggests that cell death of hair cells and proliferation of mantle supporting cells occurs as part of normal development, creating constant turnover of hair cells. We show that the caspase inhibitor zVADfmk reduces cell death while the aminoglycoside neomycin specifically induces an increased amount of cell death in the central population of cells. Both of these treatments affect the rate of proliferation of the peripheral subpopulation of cells in the neuromast suggesting that a feedback mechanism occurs regulating cell death and proliferation. We propose that the dying population of cells are hair cells and the proliferating cells are 'mantle' supporting cells, which is in agreement with previous observations suggesting that supporting cells can give rise to hair cells following hair cell death.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping