|ZFIN ID: ZDB-PUB-000201-17|
Transgenic zebrafish for detecting mutations caused by compounds in aquatic environments
Ananuma, K., Takeda, H., Amanuma, H., and Aoki, Y.
|Source:||Nat. Biotechnol. 18(1): 62-65 (Journal)|
|Registered Authors:||Amanuma, Kimiko, Aoki, Yasunobu, Takeda, Hiroyuki|
|Keywords:||transgenic zebrafish; mutation assay; rpsL gene; N-ethyl-N-nitrosourea; benzo[a]pyrene; 2-amino-3,8-dimethylimidazo[4,5-f]-; quinoxaline; aquatic environments|
|PubMed:||10625393 Full text @ Nat. Biotechnol.|
Ananuma, K., Takeda, H., Amanuma, H., and Aoki, Y. (2000) Transgenic zebrafish for detecting mutations caused by compounds in aquatic environments. Nat. Biotechnol.. 18(1):62-65.
ABSTRACTWe have established a transgenic zebrafish line carrying a shuttle vector plasmid (pML4) for detecting mutagens in aquatic environments. The plasmid contains the rpsL gene of Escherichia coli as a mutational target gene, and the kanamycin-resistance gene for recovering the plasmid from the chromosomal DNA. To evaluate the system, we treated embryos of the transgenic fish with N-ethyl-N-nitrosourea (ENU), which induces a dose-dependent increase in the mutation frequency of the target gene. The mutation spectrum was consistent with the proposed mechanism of ENU mutagenesis. Similarly, treating the embryos with benzo[a]pyrene or 2-amino-3, 8-dimethylimidazo[4,5- f]quinoxaline, which are found in naturally polluted water, significantly increased the frequency of mutations in the target gene.