ZFIN ID: ZDB-PUB-000201-17
Transgenic zebrafish for detecting mutations caused by compounds in aquatic environments
Ananuma, K., Takeda, H., Amanuma, H., and Aoki, Y.
Date: 2000
Source: Nat. Biotechnol.   18(1): 62-65 (Journal)
Registered Authors: Amanuma, Kimiko, Aoki, Yasunobu, Takeda, Hiroyuki
Keywords: transgenic zebrafish; mutation assay; rpsL gene; N-ethyl-N-nitrosourea; benzo[a]pyrene; 2-amino-3,8-dimethylimidazo[4,5-f]-; quinoxaline; aquatic environments
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Benzo(a)pyrene/toxicity
  • DNA Mutational Analysis
  • Dose-Response Relationship, Drug
  • Ethylnitrosourea/toxicity
  • Female
  • Gene Dosage
  • Genetic Vectors/genetics
  • Male
  • Mutagenesis/drug effects
  • Mutagenesis/genetics
  • Mutagenicity Tests
  • Mutagens/analysis*
  • Mutagens/toxicity*
  • Mutation*
  • Quinoxalines/toxicity
  • Transgenes/genetics*
  • Water Pollutants, Chemical/analysis*
  • Water Pollutants, Chemical/toxicity*
  • Zebrafish/embryology
  • Zebrafish/genetics*
PubMed: 10625393 Full text @ Nat. Biotechnol.
We have established a transgenic zebrafish line carrying a shuttle vector plasmid (pML4) for detecting mutagens in aquatic environments. The plasmid contains the rpsL gene of Escherichia coli as a mutational target gene, and the kanamycin-resistance gene for recovering the plasmid from the chromosomal DNA. To evaluate the system, we treated embryos of the transgenic fish with N-ethyl-N-nitrosourea (ENU), which induces a dose-dependent increase in the mutation frequency of the target gene. The mutation spectrum was consistent with the proposed mechanism of ENU mutagenesis. Similarly, treating the embryos with benzo[a]pyrene or 2-amino-3, 8-dimethylimidazo[4,5- f]quinoxaline, which are found in naturally polluted water, significantly increased the frequency of mutations in the target gene.