ZFIN ID: ZDB-PERS-960805-463
Riley, Bruce
Email: briley@mail.bio.tamu.edu
URL:
Affiliation: Riley Lab
Address: Department of Biology Texas A&M University 118A Biological Sciences Building 3258 TAMUS College Station, TX 77843-3258 USA
Country: United States
Phone: (979) 845-6494
Fax: (979) 845-2891
Orcid ID:


BIOGRAPHY AND RESEARCH INTERESTS
For most studies we use a phenotype-to-gene strategy: Randomly induced mutations are propagated to determine whether carriers display developmental defects. Mutations of interest are then genetically mapped, and eventually cloned, using PCR-based technology. We are also using gene-to-phenotype ('reverse genetics') strategies to functionally disrupt previously cloned genes. With either strategy, an important goal is to phenotypically analyze mutant embryos to establish the cellular and molecular bases for their developmental defects. Currently, the lab is focused on analysis of mutations that perturb hindbrain and inner ear development. Both organ systems are highly conserved among vertebrates and are essential for normal embryonic development and survival. Specific molecules and pathways of interest include Delta-Notch, FGF, and Wnt signaling, as well as members of the muscle segment homeobox (msx) and paired box (pax) families of transcription factors.


PUBLICATIONS
Kantarci, H., Gerberding, A., Riley, B.B. (2016) Spemann organizer gene Goosecoid promotes delamination of neuroblasts from the otic vesicle. Proceedings of the National Academy of Sciences of the United States of America. 113(44):E6840-E6848
Kantarci, H., Edlund, R.K., Groves, A.K., Riley, B.B. (2015) Tfap2a Promotes Specification and Maturation of Neurons in the Inner Ear through Modulation of Bmp, Fgf and Notch Signaling. PLoS Genetics. 11:e1005037
Maulding, K., Padanad, M.S., Dong, J., Riley, B.B. (2014) Mesodermal Fgf10b cooperates with other Fgfs during induction of otic and epibranchial placodes in zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists. 243(10):1275-85
Edlund, R.K., Ohyama, T., Kantarci, H., Riley, B.B., Groves, A.K. (2014) Foxi transcription factors promote pharyngeal arch development by regulating formation of FGF signaling centers. Developmental Biology. 390:1-13
Bhat, N., Kwon, H.J., and Riley, B.B. (2013) A gene network that coordinates preplacodal competence and neural crest specification in zebrafish. Developmental Biology. 373(1):107-117
Padanad, M.S., Bhat, N., Guo, B., and Riley, B.B. (2012) Conditions that influence the response to Fgf during otic placode induction. Developmental Biology. 364(1):1-10
Vemaraju, S., Kantarci, H., Padanad, M.S., and Riley, B.B. (2012) A spatial and temporal gradient of fgf differentially regulates distinct stages of neural development in the zebrafish inner ear. PLoS Genetics. 8(11):e1003068
Sweet, E.M., Vemaraju, S., and Riley, B.B. (2011) Sox2 and Fgf interact with Atoh1 to promote sensory competence throughout the zebrafish inner ear. Developmental Biology. 358(1):113-21
Padanad, M.S., and Riley, B.B. (2011) Pax2/8 proteins coordinate sequential induction of otic and epibranchial placodes through differential regulation of foxi1, sox3 and fgf24. Developmental Biology. 351(1):90-98
Bhat, N., and Riley, B.B. (2011) Integrin-α5 Coordinates Assembly of Posterior Cranial Placodes in Zebrafish and Enhances Fgf-Dependent Regulation of Otic/Epibranchial Cells. PLoS One. 6(12):e27778
Kwon, H.J., Bhat, N., Sweet, E.M., Cornell, R.A., and Riley, B.B. (2010) Identification of early requirements for preplacodal ectoderm and sensory organ development. PLoS Genetics. 6(9):pii: e1001133
Manohar, M., Mei, H., Franklin, A.J., Sweet, E.M., Shigaki, T., Riley, B.B., Macdiarmid, C.W., and Hirschi, K.D. (2010) A Zebrafish (Danio rerio) Endomembrane Antiporter Similar to a Yeast Cation/H+ Transporter is Required for Neural Crest Development. Biochemistry. 49(31):6557-6566
Riley, B.B., Sweet, E.M., Heck, R., Evans, A., McFarland, K.N., Warga, R.M., and Kane, D.A. (2010) Characterization of harpy/Rca1/emi1 mutants: patterning in the absence of cell division. Developmental dynamics : an official publication of the American Association of Anatomists. 239(3):828-843
Millimaki, B.B., Sweet, E.M., and Riley, B.B. (2010) Sox2 is required for maintenance and regeneration, but not initial development, of hair cells in the zebrafish inner ear. Developmental Biology. 338(2):262-269
Kwon, H.J., and Riley, B.B. (2009) Mesendodermal signals required for otic induction: Bmp-antagonists cooperate with Fgf and can facilitate formation of ectopic otic tissue. Developmental dynamics : an official publication of the American Association of Anatomists. 238(6):1582-1594
Petko, J.A., Millimaki, B.B., Canfield, V.A., Riley, B.B., and Levenson, R. (2008) Otoc1: A novel otoconin-90 ortholog required for otolith mineralization in zebrafish. Developmental Neurobiology. 68(2):209-222
Millimaki, B.B., Sweet, E.M., Dhason, M.S., and Riley, B.B. (2007) Zebrafish atoh1 genes: classic proneural activity in the inner ear and regulation by Fgf and Notch. Development (Cambridge, England). 134(2):295-305
Kwak, S.J., Vemaraju, S., Moorman, S.J., Zeddies, D., Popper, A.N., and Riley, B.B. (2006) Zebrafish pax5 regulates development of the utricular macula and vestibular function. Developmental dynamics : an official publication of the American Association of Anatomists. 235(11):3026-3038
Phillips, B.T., Kwon, H.J., Melton, C., Houghtaling, P., Fritz, A., and Riley, B.B. (2006) Zebrafish msxB, msxC and msxE function together to refine the neural-nonneural border and regulate cranial placodes and neural crest development. Developmental Biology. 294(2):376-390
Mackereth, M.D., Kwak, S.J., Fritz, A., and Riley, B.B. (2005) Zebrafish pax8 is required for otic placode induction and plays a redundant role with Pax2 genes in the maintenance of the otic placode. Development (Cambridge, England). 132(2):371-382
Riley, B.B., Chiang, M.Y., Storch, E.M., Heck, R., Buckles, G.R., and Lekven, A.C. (2004) Rhombomere boundaries are Wnt signaling centers that regulate metameric patterning in the zebrafish hindbrain. Developmental dynamics : an official publication of the American Association of Anatomists. 231(2):278-291
Phillips, B.T., Storch, E.M., Lekven, A.C., and Riley, B.B. (2004) A direct role for Fgf but not Wnt in otic placode induction. Development (Cambridge, England). 131(4):923-931
Riley, B.B. and Phillips, B.T. (2003) Ringing in the new ear: resolution of cell interactions in otic development. Developmental Biology. 261(2):289-312
Kwak, S.-J., Phillips, B.T., Heck R., and Riley, B.B. (2002) An expanded domain of fgf3 expression in the hindbrain of zebrafish valentino mutants results in mis-patterning of the otic vesicle. Development (Cambridge, England). 129(22):5279-5287
Whitfield, T.T., Riley, B.B., Chiang, M.-Y., and Phillips, B. (2002) Development of the zebrafish inner ear. Developmental dynamics : an official publication of the American Association of Anatomists. 223(4):427-458
Phillips, B.T., Bolding, K., and Riley, B.B. (2001) Zebrafish fgf3 and fgf8 encode redundant functions required for otic placode induction. Developmental Biology. 235(2):351-365
Riley, B.B., Chiang, M.Y., Lekven, A.C., Moon, R.T. (2001) A signaling network required to maintain rhombomere boundaries as organizing centers in the zebrafish hindbrain. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 15(5):A742
Riley, B.B. and Moorman, S.J. (2000) Development of utricular otoliths, but not saccular otoliths, is necessary for vestibular function and survival in zebrafish. Journal of neurobiology. 43(4):329-337
Riley, B.B., Chiang, M.Y., Farmer, L., and Heck, R. (1999) The deltaA gene of zebrafish mediates lateral inhibition of hair cells in the inner ear and is regulated by pax2.1. Development (Cambridge, England). 126(24):5669-5678
Appel, B., Fritz, A., Westerfield, M., Grunwald, D.J., Eisen, J.S., and Riley, B.B. (1999) Delta-mediated specification of midline cell fates in zebrafish embryos. Current biology : CB. 9:247-256
Mendonsa, E.S. and Riley, B.B. (1999) Genetic analysis of tissue interactions required for otic placode induction in the zebrafish. Developmental Biology. 206:100-112
Riley, B.B., Zhu, C., Janetopoulos, C., and Aufderheide, K.J. (1997) A critical period of ear development controlled by distinct populations of ciliated cells in the zebrafish. Developmental Biology. 191(2):191-201
Riley, B.B. and Grunwald, D.J. (1996) A mutation in zebrafish affecting a localized cellular function required for normal ear development. Developmental Biology. 179(2):427-435
Riley, B.B. and Grunwald, D.J. (1995) Efficient induction of point mutations allowing recovery of specific locus mutations in zebrafish. Proc. Natl. Acad. Sci. USA. 92:5997-6001

NON-ZEBRAFISH PUBLICATIONS