ZFIN ID: ZDB-PERS-960805-277
Joly, Jean-Stephane
Email: joly@iaf.cnrs-gif.fr
URL: http://www.inaf.cnrs-gif.fr/ned/equipe08_eng/accueil_08.html
Affiliation: Joly Lab
Address: Equipe INRA 'Morphogenèse du système nerveux des Chordés' UPR 3294 NED Institut de Neurosciences A. Fessard CNRS 1 Avenue de la Terrasse GIF-SUR -YVETTE 91 198 France
Country: France
Phone: 33-1-6982-3431
Fax: 33-1-6982-3447
Orcid ID:

The optic tectum grows continuously by the addition of cells produced in a proliferative zone located at the margin of the structure (Nguyen et al., 1999). This proliferative zone therefore exhibits, in dorsal view, a quite characteristic annular shape. This peculiar mode of growth allows to determine easily and quickly whether a gene is expressed in proliferating cells (marginal zone) of in differentiared cells (central zone). We performed several in situ screens (Nguyen et al., 2001, Mechanisms of Development, 107, 55-67 ; Deyts et al., 2005, Developmental Dynamics, 234, 698-708) and isolated a sizeable number of previously uncharacterized genes, which could represent novel regulators of cell proliferation. In addition, we detected a third class of genes, expressed in an open ring of cells located between the marginal (proliferative) zone and the central (differentiation) zone (see Fig. 1). These genes correspond to negative regulators of cell proliferation (cell cycle arrest genes). More recently, we identified a zone of relatively slow cycling progenitors in the peripheral midbrain layer (PML), bordering the tectum, which have molecular features similar to drosophila neuroblasts and mammalian stem cells. This population of stem cells should become a pioneering cellular model for the study of neural stem cell.

Project 1:

Function of tumor suppressor genes in the development of the medaka optic tectum. Person in charge: Franck Bourrat

Project 2 :

Neural stem cells and regulators of pluripotency in the zebrafish peripheral midbrain layer (PML). Persons in charge: Françoise Jamen, Jean-Michel Hermel and Jean-Stéphane Joly.

Lindsey, B.W., Hall, Z.J., Heuzé, A., Joly, J.S., Tropepe, V., Kaslin, J. (2018) The role of neuro-epithelial-like and radial-glial stem and progenitor cells in development, plasticity, and repair. Prog. Neurobiol.. 170:99-114
Bouffard, S., Dambroise, E., Brombin, A., Lempereur, S., Hatin, I., Simion, M., Corre, R., Bourrat, F., Joly, J.S., Jamen, F. (2018) Fibrillarin is essential for S-phase progression and neuronal differentiation in zebrafish dorsal midbrain and retina. Developmental Biology. 437(1):1-16
Passoni, G., Langevin, C., Palha, N., Mounce, B.C., Briolat, V., Affaticati, P., De Job, E., Joly, J.S., Vignuzzi, M., Saleh, M.C., Herbomel, P., Boudinot, P., Levraud, J.P. (2017) Imaging of viral neuroinvasion in the zebrafish reveals that Sindbis and chikungunya viruses favour different entry routes. Disease models & mechanisms. 10(7):847-857
Frétaud, M., Rivière, L., Job, É., Gay, S., Lareyre, J.J., Joly, J.S., Affaticati, P., Thermes, V. (2017) High-resolution 3D imaging of whole organ after clearing: taking a new look at the zebrafish testis. Scientific Reports. 7:43012
Mayrhofer, M., Gourain, V., Reischl, M., Affaticati, P., Jenett, A., Joly, J.S., Benelli, M., Demichelis, F., Poliani, P.L., Sieger, D., Mione, M. (2017) A novel brain tumour model in zebrafish reveals the role of YAP activation in MAPK/PI3K induced malignant growth. Disease models & mechanisms. 10(1):15-28
Benítez-Santana, T., Simion, M., Orraze, G., Médale, F., Joly, J.S. (2017) Effect of nutrient availability on progenitor cells in zebrafish (Danio rerio). Developmental Neurobiology. 77(1):26-38
Haeussler, M., Schönig, K., Eckert, H., Eschstruth, A., Mianné, J., Renaud, J.B., Schneider-Maunoury, S., Shkumatava, A., Teboul, L., Kent, J., Joly, J.S., Concordet, J.P. (2016) Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection tool CRISPOR. Genome biology. 17:148
Dambroise, E., Monnier, L., Ruisheng, L., Aguilaniu, H., Joly, J.S., Tricoire, H., Rera, M. (2016) Two phases of aging separated by the Smurf transition as a public path to death. Scientific Reports. 6:23523
Radev, Z., Hermel, J.M., Elipot, Y., Bretaud, S., Arnould, S., Duchateau, P., Ruggiero, F., Joly, J.S., Sohm, F. (2015) A TALEN-Exon Skipping Design for a Bethlem Myopathy Model in Zebrafish. PLoS One. 10:e0133986
Recher, G., Jouralet, J., Brombin, A., Heuzé, A., Mugniery, E., Hermel, J.M., Desnoulez, S., Savy, T., Herbomel, P., Bourrat, F., Peyriéras, N., Jamen, F., and Joly, J.S. (2013) Zebrafish midbrain slow-amplifying progenitors exhibit high levels of transcripts for nucleotide and ribosome biogenesis. Development (Cambridge, England). 140(24):4860-9
Gautier, A., Sohm, F., Joly, J.S., Le Gac, F., and Lareyre, J.J. (2011) The Proximal Promoter Region of the Zebrafish gsdf Gene Is Sufficient to Mimic the Spatio-Temporal Expression Pattern of the Endogenous Gene in Sertoli and Granulosa Cells. Biology of reproduction. 85(6):1240-51
Gibert, Y., Bernard, L., Debiais-Thibaud, M., Bourrat, F., Joly, J.S., Pottin, K., Meyer, A., Retaux, S., Stock, D.W., Jackman, W.R., Seritrakul, P., Begemann, G., and Laudet, V. (2010) Formation of oral and pharyngeal dentition in teleosts depends on differential recruitment of retinoic acid signaling. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 24(9):3298-3309
Grabher, C., Joly, J.S., and Wittbrodt, J. (2004) Highly efficient zebrafish transgenesis mediated by the meganuclease I-SceI. The Zebrafish: Genetics, Genomics and Informatics, 2nd ed., Methods Cell Biol.. 77:381-401
Thermes, V., Grabher, C., Ristoratore, F., Bourrat, F., Choulika, A., Wittbrodt, J., and Joly, J.S. (2002) I-SceI meganuclease mediates highly efficient transgenesis in fish. Mechanisms of Development. 118(1-2):91-98
Deschet, K., Thermes, V., and Joly, J.S. (2001) The transgenesis among the fishes. Bull. Soc. Zool. France. 126(3):255-268
Deschet, K., Bourrat, F., Ristoratore, F., Chourrout, D., and Joly, J.S. (1999) Expression of the medaka (Oryzias latipes) Ol-Rx3 paired-like gene in two diencephalic derivatives, the eye and the hypothalamus. Mechanisms of Development. 83(1-2):179-182
Postlethwait, J.H, Yan, Y.-L., Gates, M.A., Horne, S., Amores, A., Brownlie, A., Donovan, A., Egan, E.S., Force, A., Gong, Z., Goutel, C., Fritz, A., Kelsh, R., Knapik, E., Liao, E., Paw, B., Ransom, D., Singer, A., Thomson, M., Abduljabbar, T.S., Yelick, P., Beier, D., Joly, J.-S., Larhammar, D., Rosa, F., Westerfield, M., Zon, L.I., Johnson, S.L., and Talbot, W.S. (1998) Vertebrate genome evolution and the zebrafish gene map. Nature Genetics. 18:345-349
Barro, O., Vriz, S., Joly, J.S., Joly, C., Condamine, H., and Boulekbache, H. (1995) Widespread expression of the eve1 gene in zebrafish embryos affects the anterior-posterior axis pattern. Developmental genetics. 17:117-128
Joly, J.S., Joly, C., Schulte-Merker, S., Boulekbache, H., and Condamine, H. (1993) The ventral and posterior expression of the zebrafish homeobox gene eve1 is perturbed in dorsalized and mutant embryos. Development (Cambridge, England). 119:1261-1275
Joly, J.S., Maury, M., Joly, C., and Boulekbache, H. (1993) Ventral and posterior expression of the homeobox gene eve1 in zebrafish (Brachydanio rerio) is repressed in dorsalized embryos. Comptes rendus des seances de la Societe de biologie et de ses filiales. 187:356-363
Joly, J.S., Maury, M., Joly, C., Duprey, P., Boulekbache, H., and Condamine, H. (1992) Expression of a zebrafish caudal homeobox gene correlates with the establishment of posterior cell lineages at gastrulation. Differentiation; research in biological diversity. 50:75-87
Joly, J.S., Maury, M., Joly, C., Boulekbache, H., and Condamine, H. (1991) Towards vertebrate developmental genetics structural and functional conservation of an even-skipped gene in fish, amphibian, and mammal. Bull. Soc. Zool. Fr. Evol. Zool.. 116:123-128

Brombin A, Grossier JP, Heuzé A, Radev Z, Bourrat F, Joly JS, Jamen F.
Genome-wide analysis of the POU genes in medaka, focusing on expression in the optic tectum. Dev Dyn. 2011 Oct;240(10):2354-63. doi: 10.1002/dvdy.22727. Epub 2011 Aug 26. PubMed PMID: 21932313.

Haeussler M, Joly JS. When needles look like hay: how to find tissue-specific enhancers in model organism genomes. Dev Biol. 2011 Feb 15;350(2):239-54.

Tassy O, Dauga D, Daian F, Sobral D, Robin F, Khoueiry P, Salgado D, Fox V, Caillol D, Schiappa R, Laporte B, Rios A, Luxardi G, Kusakabe T, Joly JS, Darras S, Christiaen L, Contensin M, Auger H, Lamy C, Hudson C, Rothbächer U, Gilchrist MJ, Makabe KW, Hotta K, Fujiwara S, Satoh N, Satou Y, Lemaire P. The ANISEED database: digital representation, formalization, and elucidation of a chordate developmental program. Genome Res. 2010 Oct;20(10):1459-68.

Alunni A, Hermel JM, Heuzé A, Bourrat F, Jamen F, Joly JS. Evidence for neural stem cells in the medaka optic tectum proliferation zones. Dev Neurobiol. 2010 Sep;70(10):693-713.

Haeussler M, Jaszczyszyn Y, Christiaen L, Joly JS. A cis-regulatory signature for chordate anterior neuroectodermal genes. PLoS Genet. 2010 Apr15;6(4):e1000912.

Auger H, Sasakura Y, Joly JS, Jeffery WR. Regeneration of oral siphon pigment organs in the ascidian Ciona intestinalis. Dev Biol. 2010 Mar 15;339(2):374-89.

Auger H, Lamy C, Haeussler M, Khoueiry P, Lemaire P, Joly JS. Similar regulatory logic in Ciona intestinalis for two Wnt pathway modulators, ROR and SFRP-1/5. Dev Biol. 2009 May 15;329(2):364-73.

Dahlberg C, Auger H, Dupont S, Sasakura Y, Thorndyke M, Joly JS. Refining the Ciona intestinalis model of central nervous system regeneration. PLoS One. 2009;4(2):e4458.