ZFIN ID: ZDB-PERS-090602-1
Miller, Yury
Email: yumiller@ucsd.edu
Affiliation: Yury Miller Lab
Address: Associate Professor of Medicine University of California, San Diego 9500 Gilman Drive La Jolla, CA 92093-0682 USA
Country: United States
Phone: 858-822-5771
Fax: 858-534-2005

Miller’s laboratory studies inflammatory mechanisms involved in the development of atherosclerosis. A new exciting direction in our research is studying the processes of vascular inflammation in zebrafish. The optical transparency of zebrafish larvae enables monitoring vascular lipid and macrophage accumulation in real time in a live animal. This opens endless possibilities for in vivo functional studies of certain processes of atherogenesis. We are making new transgenic zebrafish for this study. A recent advance is the development of a zebrafish with conditional expression of human oxidation-specific antibody conjugated with GFP. Upon heat shock, these zebrafish express the antibody and the GFP signal is detected to monitor in vivo accumulation of oxidized lipid in the blood vessel wall.

Fang, L., Miller, Y.I. (2019) Regulation of lipid rafts, angiogenesis and inflammation by AIBP. Current opinion in lipidology. 30(3):218-223
Gu, Q., Yang, X., Lv, J., Zhang, J., Xia, B., Kim, J.D., Wang, R., Xiong, F., Meng, S., Clements, T.P., Tandon, B., Wagner, D.S., Diaz, M.F., Wenzel, P.L., Miller, Y.I., Traver, D., Cooke, J.P., Li, W., Zon, L.I., Chen, K., Bai, Y., Fang, L. (2019) AIBP-mediated cholesterol efflux instructs hematopoietic stem and progenitor cell fate. Science (New York, N.Y.). 363:1085-1088
Wang, H., Lapek, J., Fujimura, K., Strnadel, J., Liu, B., Gonzalez, D.J., Zhang, W., Watson, F., Yu, V., Liu, C., Melo, C.M., Miller, Y.I., Elliott, K.C., Cheresh, D.A., Klemke, R.L. (2018) Pseudopodium-enriched atypical kinase 1 mediates angiogenesis by modulating GATA2-dependent VEGFR2 transcription. Cell discovery. 4:26
Savchenko, A., Cherkas, V., Liu, C., Braun, G.B., Kleschevnikov, A., Miller, Y.I., Molokanova, E. (2018) Graphene biointerfaces for optical stimulation of cells. Science advances. 4:eaat0351
Liu, C., Han, T., Stachura, D.L., Wang, H., Vaisman, B.L., Kim, J., Klemke, R.L., Remaley, A.T., Rana, T.M., Traver, D., Miller, Y.I. (2018) Lipoprotein lipase regulates hematopoietic stem progenitor cell maintenance through DHA supply. Nature communications. 9:1310
Schneider, D.A., Choi, S.H., Agatisa-Boyle, C., Zhu, L., Kim, J., Pattison, J., Sears, D.D., Gordts, P.L.S.M., Fang, L., Miller, Y.I. (2018) AIBP protects against metabolic abnormalities and atherosclerosis. Journal of Lipid Research. 59(5):854-863
Liu, C., Kim, Y.S., Kim, J., Pattison, J., Kamaid, A., Miller, Y.I. (2017) Modeling hypercholesterolemia and vascular lipid accumulation in LDL receptor mutant zebrafish. Journal of Lipid Research. 59(2):391-399
Meng, S., Mao, R., Gu, Q., Araujo-Gutierrez, R., Kumar, S., Yan, Q., Almazan, F., Youker, K.A., Fu, Y., Pownall, H.J., Cooke, J.P., Miller, Y.I., Fang, L. (2017) AIBP Limits Angiogenesis Through γ-Secretase-Mediated Upregulation of Notch Signaling. Circulation research. 120(11):1727-1739
Liu, C., Gaudet, D., Miller, Y.I. (2017) Deficient Cholesterol Esterification in Plasma of apoc2 Knockout Zebrafish and Familial Chylomicronemia Patients. PLoS One. 12:e0169939
Yang, H., Fang, L., Zhan, R., Hegarty, J.M., Ren, J., Hsiai, T.K., Gleeson, J.G., Miller, Y.I., Trejo, J., Chi, N.C. (2015) Polo-like kinase 2 regulates angiogenic sprouting and blood vessel development. Developmental Biology. 404(2):49-60
Liu, C., Gates, K.P., Fang, L., Amar, M.J., Schneider, D.A., Geng, H., Huang, W., Kim, J., Pattison, J., Zhang, J., Witztum, J.L., Remaley, A.T., Dong, P.D., Miller, Y.I. (2015) Apoc2 loss-of-function zebrafish mutant as a genetic model of hyperlipidemia. Disease models & mechanisms. 8(8):989-98
Chen, Z., Wen, L., Martin, M., Hsu, C., Fang, L., Lin, F., Lin, T., Geary, M.J., Geary, G., Zhao, Y., Johnson, D.A., Chen, J., Lin, S., Chien, S., Huang, H., Miller, Y.I., Huang, P., Shyy, J.Y. (2015) Oxidative Stress Activates Endothelial Innate Immunity via Sterol Regulatory Element Binding Protein 2 (SREBP2) Transactivation of MiRNA-92a. Circulation. 131(9):805-14
Fang, L., Liu, C., and Miller, Y.I. (2014) Zebrafish models of dyslipidemia: relevance to atherosclerosis and angiogenesis. Translational research : the journal of laboratory and clinical medicine. 163(2):99-108
Fang, L., and Miller, Y.I. (2013) Targeted cholesterol efflux. Cell cycle (Georgetown, Tex.). 12(21):3345-6
Lee, J., Moghadam, M.E., Kung, E., Cao, H., Beebe, T., Miller, Y., Roman, B.L., Lien, C.L., Chi, N.C., Marsden, A.L., and Hsiai, T.K. (2013) Moving domain computational fluid dynamics to interface with an embryonic model of cardiac morphogenesis. PLoS One. 8(8):e72924
Fang, L., Choi, S.H., Baek, J.S., Liu, C., Almazan, F., Ulrich, F., Wiesner, P., Taleb, A., Deer, E., Pattison, J., Torres-Vázquez, J., Li, A.C., and Miller, Y.I. (2013) Control of angiogenesis by AIBP-mediated cholesterol efflux. Nature. 498(7452):118-122
Fang, L., and Miller, Y.I. (2012) Emerging applications for zebrafish as a model organism to study oxidative mechanisms and their roles in inflammation and vascular accumulation of oxidized lipids. Free radical biology & medicine. 53(7):1411-1420
Baek, J.S., Fang, L., Li, A.C., and Miller, Y.I. (2012) Ezetimibe and simvastatin reduce cholesterol levels in zebrafish larvae fed a high-cholesterol diet. Cholesterol. 2012:564705
Fang, L., Green, S.R., Baek, J.S., Lee, S.H., Ellett, F., Deer, E., Lieschke, G.J., Witztum, J.L., Tsimikas, S., and Miller, Y.I. (2011) In vivo visualization and attenuation of oxidized lipid accumulation in hypercholesterolemic zebrafish. J. Clin. Invest.. 121(12):4861-9
Fang, L., Harkewicz, R., Hartvigsen, K., Wiesner, P., Choi, S.H., Almazan, F., Pattison, J., Deer, E., Sayaphupha, T., Dennis, E.A., Witztum, J.L., Tsimikas, S., and Miller, Y.I. (2010) Oxidized cholesteryl esters and phospholipids in zebrafish larvae fed a high cholesterol diet: macrophage binding and activation.. The Journal of biological chemistry. 285(42):32343-32351
Miller, Y.I., Choi, S.H., Fang, L., and Harkewicz, R. (2009) Toll-like receptor-4 and lipoprotein accumulation in macrophages. Trends in cardiovascular medicine. 19(7):227-232
Stoletov, K., Fang, L., Choi, S.H., Hartvigsen, K., Hansen, L.F., Hall, C., Pattison, J., Juliano, J., Miller, E.R., Almazan, F., Crosier, P., Witztum, J.L., Klemke, R.L., and Miller, Y.I. (2009) Vascular Lipid Accumulation, Lipoprotein Oxidation, and Macrophage Lipid Uptake in Hypercholesterolemic Zebrafish. Circulation research. 104(8):952-960