ZFIN ID: ZDB-PERS-060629-1
Kamachi, Yusuke
Email: kamachi.yusuke@kochi-tech.ac.jp
URL:
Affiliation: Kamachi Lab
Address: School of Environmental Science and Engineering Kochi University of Technology 185 Miyanokuchi, Tosayamada-cho, Kami Kochi 782-8502, Japan
Country: Japan
Phone: 81-887-57-2510
Fax:
ORCID ID:


BIOGRAPHY AND RESEARCH INTERESTS


PUBLICATIONS
Okada, K., Aoki, K., Tabei, T., Sugio, K., Imai, K., Bonkohara, Y., Kamachi, Y. (2022) Key sequence features of CRISPR RNA for dual-guide CRISPR-Cas9 ribonucleoprotein complexes assembled with wild-type or HiFi Cas9. Nucleic acids research. 50(5):2854-2871
Ranawakage, D.C., Okada, K., Sugio, K., Kawaguchi, Y., Kuninobu-Bonkohara, Y., Takada, T., Kamachi, Y. (2021) Efficient CRISPR-Cas9-Mediated Knock-In of Composite Tags in Zebrafish Using Long ssDNA as a Donor. Frontiers in cell and developmental biology. 8:598634
Okamoto, Y., Nishimura, N., Matsuda, K., Ranawakage, D.C., Kamachi, Y., Kondoh, H., Uchikawa, M. (2018) Cooperation of Sall4 and Sox8 transcription factors in the regulation of the chicken Sox3 gene during otic placode development. Development, growth & differentiation. 60(3):133-145
Iwafuchi-Doi, M., Yoshida, Y., Onichtchouk, D., Leichsenring, M., Driever, W., Takemoto, T., Uchikawa, M., Kamachi, Y., and Kondoh, H. (2011) The Pou5f1/Pou3f-dependent but SoxB-independent regulation of conserved enhancer N2 initiates Sox2 expression during epiblast to neural plate stages in vertebrates. Developmental Biology. 352(2):354-366
Okuda, Y., Ogura, E., Kondoh, H., and Kamachi, Y. (2010) B1 SOX coordinate cell specification with patterning and morphogenesis in the early zebrafish embryo. PLoS Genetics. 6:e1000936
Ogura, E., Okuda, Y., Kondoh, H., and Kamachi, Y. (2009) Adaptation of GAL4 activators for GAL4 enhancer trapping in zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 238(3):641-655
Kamachi, Y., Okuda, Y., and Kondoh, H. (2008) Quantitative assessment of the knockdown efficiency of morpholino antisense oligonucleotides in zebrafish embryos using a luciferase assay. Genesis (New York, N.Y. : 2000). 46(1):1-7
Okuda, Y., Yoda, H., Uchikawa, M., Furutani-Seiki, M., Takeda, H., Kondoh, H., Kamachi, Y. (2006) Comparative genomic and expression analysis of group B1 sox genes in zebrafish indicates their diversification during vertebrate evolution. Developmental Dynamics : an official publication of the American Association of Anatomists. 235(3):811-825

NON-ZEBRAFISH PUBLICATIONS
Inoue, M., Kamachi, Y., Matsunami, H., Imada, K., Uchikawa, M. and Kondoh, H. (2007). PAX6 and SOX2-dependent regulation of the Sox2 enhancer N-3 involved in embryonic visual system development. Genes Cells 12, 1049-1061.

Sato, N., Kamachi, Y., Kondoh, H., Shima, Y., Morohashi, K., Horikawa, R. and Ogata, T. (2007). Hypogonadotropic hypogonadism in an adult female with a heterozygous hypomorphic mutation of SOX2. Eur J Endocrinol 156, 167-171.

Yasuhara, N., Shibazaki, N., Tanaka, S., Nagai, M., Kamikawa, Y., Oe, S., Asally, M., Kamachi, Y., Kondoh, H. and Yoneda, Y. (2007). Triggering neural differentiation of ES cells by subtype switching of importin-alpha. Nat Cell Biol 9, 72-79.

Takemoto, T., Uchikawa, M., Kamachi, Y. and Kondoh, H. (2006). Convergence of Wnt and FGF signals in the genesis of posterior neural plate through activation of the Sox2 enhancer N-1. Development 133, 297-306.

Matsumata, M., Uchikawa, M., Kamachi, Y., and Kondoh, H. (2005) Multiple N-cadherin enhancers identified by systematic functional screening indicate its Group B1 SOX-dependent regulation in neural and placodal development. Dev. Biol., 286, 601-617.

Blanco, J., Girard, F., Kamachi, Y., Kondoh, H., and Gehring, WJ. (2005) Functional analysis of the chicken delta1-crystallin enhancer activity in Drosophila reveals remarkable evolutionary conservation between chicken and fly. Development. 132, 1895-1905.

Kondoh, H., Uchikawa, M., and Kamachi, Y. (2004) Interplay of Pax6 and SOX2 in lens development as a paradigm of genetic switch mechanisms for cell differentiation. Int. J. Dev. Biol. 48. 819-827.

Tanaka, S., Kamachi, Y., Tanouchi, A., Hamada, H., Jing N., and Kondoh, H. (2004) Interplay of SOX and POU factors in regulation of the Nestin gene in neural primordial cells. Mol. Cell. Biol., 24, 8834-8846.

Uchikawa, M., Takemoto, T., Kamachi, Y. and Kondoh, H. (2004) Efficient identification of regulatory sequences in the chicken genome by a powerful combination of embryo electroporation and genome comparison. Mech. Dev. 121, 145-1158.

Uchikawa, M., Ishida, Y., Takemoto, T., Kamachi, Y. and Kondoh, H. (2003) Functional analysis of chicken Sox2 enhancers highlights an array of diverse regulatory elements that are conserved in mammals. Dev. Cell, 4, 509-519.

Muta, M., Kamachi, Y., Yoshimoto, A., Higashi, Y. and Kondoh, H. (2002) Distinct roles of SOX2, Pax6 and Maf transcription factors in the regulation of lens-specific d1-crystallin enhancer. Genes to Cells, 7, 791-805.

Kamachi, Y., Uchikawa, M., Tanouchi, A., Sekido, R, and Kondoh, H. (2001) Pax6 and SOX2 form a co-DNA-binding partner complex that regulates initiation of lens development. Genes Dev. 15, 1272-1286.

Olsson, JE., Kamachi, Y., Penning, S., Muscat, GE., Kondoh, H., and Koopman P. (2001) Sox18 expression in blood vessels and feather buds during chicken embryogenesis. Gene 271, 151-158.

Kamachi, Y., Uchikawa, M. and Kondoh, H. (2000) Pairing SOX off with partners in regulation of embryonic development. Trends Genet. 16, 182-187.

Kamachi, Y., Cheah, K.S.E. and Kondoh, H. (1999) Mechanism of regulatory target selection by the SOX High-Mobility-Group domain proteins as revealed by comparison of SOX1/2/3 and SOX9. Mol. Cell. Biol. 19, 107-120.

Uchikawa, M., Kamachi, Y. and Kondoh, H. (1999) Two distinct subgroups of Group B Sox genes for transcriptional activators and repressors: their expression during embryonic organogenesis of the chicken. Mech. Dev. 84, 103-120.

Kamachi, Y., Uchikawa, M., Collignon, J., Lovell-Badge, R. and Kondoh, H. (1998) Involvement of Sox1, 2 and 3 in the early and subsequent molecular events of lens induction. Development 125, 2521-2532.

Sekido, R., Murai, K., Kamachi, Y., and Kondoh, H. (1997) Two mechanisms in the action of repressor ƒÂEF1: binding site competition with an activator and active repression. Genes Cells. 2:771-783.

Kadokawa, Y., Kusakabe, T., Kamachi, Y., Isobe, K., Kondoh, H., and Ohyama, T. (1995) A murine Thy-1.2 reporter vector containing a SV40 origin for rapid cloning and analysis of eukaryotic promoters. Gene 153:277-278.

Kamachi, Y., Sockanathan, S., Liu, Q., Breitman, M., Lovell-Badge, R. and Kondoh, H. (1995) Involvement of SOX proteins in lens-specific activation of crystallin genes. EMBO J. 14:3510-3519.

Sekido, R., Murai, K., Funahashi, J., Kamachi, Y., Fujisawa, A., Nabeshima, Y. and Kondoh, H. (1994) The ƒÂ-crystallin enhancer-binding protein ƒÂEF1 is a repressor of E2-box-mediated gene activation. Mol. Cell. Biol. 14:5692-5700.

Funahashi, J., Sekido, R., Murai, K., Kamachi, Y. and Kondoh, H. (1993) ƒÂ-crystallin enhancer binding protein ƒÂEF1 is a zinc finger- homeodomain protein implicated in postgastrulation embryogenesis. Development 119:433-446.

Kamachi, Y. and Kondoh, H. (1993) Overlapping positive and negative regulatory elements determine lens-specific activity of the ƒÂ1-crystallin enhancer. Mol. Cell. Biol. 13:5206-5215.

Funahashi, J., Kamachi, Y., Goto, K. and Kondoh, H. (1991) Identification of nuclear factor ƒÂEF1 and its binding site essential for lens-specific activity of the ƒÂ1-crystallin enhancer. Nucleic Acids Res., 19:3543-3547.

Kamachi,Y., Ogawa, E., Asano, M., Ishida, S., Murakami, Y., Satake, M., Ito, Y. and Shigesada, K. (1990) Purification of a mouse nuclear factor that binds to both the A and B cores of the polyomvirus enhancer. J.Virology 64:4808-4819.