Morpholino

MO4-mcm2

ID

ZDB-MRPHLNO-190603-1

Name

MO4-mcm2

Previous Names

None

Target

mcm2 (1)

Sequence

5' - GAAAAGTGCATCTCTCTCACCTCTC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

Splice-blocking morpholino. This morpholino contains a single base pair mismatch when blasted in Ensembl. The authors designed it against their wild-type strains.

Genome Resources

None

Target Location

Genomic Features

No data available

Expression

Gene expression in Wild Types + MO4-mcm2

Expressed Gene	Anatomy	Figures
gli1	whole organism	Fig. 3 from Casar Tena et al., 2018
ins	endocrine pancreas	Fig. 2 from Casar Tena et al., 2018
mcm2	whole organism	Fig. 1 from Casar Tena et al., 2018
myl7	atrium cardiac ventricle	Fig. 2 from Casar Tena et al., 2018
nkx2.2a	whole organism	Fig. 3 from Casar Tena et al., 2018
spaw	lateral plate mesoderm right side	Fig. 2 from Casar Tena et al., 2018

Phenotype

Phenotype resulting from MO4-mcm2

Phenotype	Fish	Figures
endocrine pancreas mislocalised, abnormal	WT + MO4-mcm2	Fig. 2 from Casar Tena et al., 2018
endocrine pancreas determination of pancreatic left/right asymmetry decreased occurrence, abnormal	WT + MO4-mcm2	Fig. 2 from Casar Tena et al., 2018
eye decreased size, abnormal	WT + MO4-mcm2	Fig. 1 from Casar Tena et al., 2018
head decreased size, abnormal	WT + MO4-mcm2	Fig. 1 from Casar Tena et al., 2018
heart edematous, abnormal	WT + MO4-mcm2	Fig. 1 from Casar Tena et al., 2018
heart heart looping decreased occurrence, abnormal	WT + MO4-mcm2	Fig. 2 , Fig. 3 from Casar Tena et al., 2018
lateral plate mesoderm determination of left/right asymmetry in lateral mesoderm decreased occurrence, abnormal	WT + MO4-mcm2	Fig. 2 from Casar Tena et al., 2018
lateral plate mesoderm right side spaw expression mislocalised, abnormal	WT + MO4-mcm2	Fig. 2 from Casar Tena et al., 2018
whole organism nkx2.2a expression decreased amount, abnormal	WT + MO4-mcm2	Fig. 3 from Casar Tena et al., 2018
whole organism gli1 expression decreased amount, abnormal	WT + MO4-mcm2	Fig. 3 from Casar Tena et al., 2018
whole organism mcm2 expression decreased amount, abnormal	WT + MO4-mcm2	Fig. 1 from Casar Tena et al., 2018
whole organism smoothened signaling pathway decreased occurrence, abnormal	WT + MO4-mcm2	Fig. 3 from Casar Tena et al., 2018

Phenotype of all Fish created by or utilizing MO4-mcm2

Phenotype	Fish	Conditions	Figures
eye decreased size, abnormal	WT + MO4-mcm2	standard conditions	Fig. 1 from Casar Tena et al., 2018
whole organism nkx2.2a expression decreased amount, abnormal	WT + MO4-mcm2	standard conditions	Fig. 3 from Casar Tena et al., 2018
whole organism mcm2 expression decreased amount, abnormal	WT + MO4-mcm2	standard conditions	Fig. 1 from Casar Tena et al., 2018
lateral plate mesoderm determination of left/right asymmetry in lateral mesoderm decreased occurrence, abnormal	WT + MO4-mcm2	standard conditions	Fig. 2 from Casar Tena et al., 2018
heart heart looping decreased occurrence, abnormal	WT + MO4-mcm2	standard conditions	Fig. 2 , Fig. 3 from Casar Tena et al., 2018
endocrine pancreas mislocalised, abnormal	WT + MO4-mcm2	standard conditions	Fig. 2 from Casar Tena et al., 2018
head decreased size, abnormal	WT + MO4-mcm2	standard conditions	Fig. 1 from Casar Tena et al., 2018
lateral plate mesoderm right side spaw expression mislocalised, abnormal	WT + MO4-mcm2	standard conditions	Fig. 2 from Casar Tena et al., 2018
endocrine pancreas determination of pancreatic left/right asymmetry decreased occurrence, abnormal	WT + MO4-mcm2	standard conditions	Fig. 2 from Casar Tena et al., 2018
heart edematous, abnormal	WT + MO4-mcm2	standard conditions	Fig. 1 from Casar Tena et al., 2018
whole organism smoothened signaling pathway decreased occurrence, abnormal	WT + MO4-mcm2	standard conditions	Fig. 3 from Casar Tena et al., 2018
whole organism gli1 expression decreased amount, abnormal	WT + MO4-mcm2	standard conditions	Fig. 3 from Casar Tena et al., 2018

Citations