ZFIN ID: ZDB-LAB-121114-2
Ishitani Lab
PI/Director: Ishitani, Tohru
Contact Person: Ishitani, Tohru
Email: ishitani@biken.osaka-u.ac.jp
URL: http://www.biken.osaka-u.ac.jp/en/laboratories/detail/52
Address: Osaka University 3-1 Yamadaoka, Suita, Osaka 565-0871
Country: Japan
Phone: +81-6-6879-8358
Fax:
Line Designation: ou


GENOMIC FEATURES ORIGINATING FROM THIS LAB
Show all 13 genomic features


STATEMENT OF RESEARCH INTERESTS
We focus especially on the cell-cell communication and behavior supporting tissue homeostasis and explore unknown molecular systems controlling embryonic development, organogenesis, regeneration, aging, and disease, using in vivo imaging, animal model genetics, molecular and cell biology, and biochemistry techniques.


LAB MEMBERS
Abe, Kota Post-Doc Haraoka, Yukinari Graduate Student Ogamino, Shohei Graduate Student
Semmy, Daniel Graduate Student Zou, Juqi Graduate Student Akieda, Yuki
Ishitani, Shizuka Mogi, Chihiro Oginuma, Masayuki


ZEBRAFISH PUBLICATIONS OF LAB MEMBERS
Zou, J., Anai, S., Ota, S., Ishitani, S., Oginuma, M., Ishitani, T. (2023) Determining zebrafish dorsal organizer size by a negative feedback loop between canonical/non-canonical Wnts and Tlr4/NFκB. Nature communications. 14:71947194
Ishitani, T. (2023) Cadherin-linked morphogen gradient actualizes robust tissue patterning. Current opinion in cell biology. 85:102275102275
Haraoka, Y., Miyake, M., Ishitani, T. (2023) Zebrafish imaging reveals hidden oncogenic-normal cell communication during primary tumorigenesis. Cell structure and function. 48(1):113-121
Kuromiya, K., Aoki, K., Ishibashi, K., Yotabun, M., Sekai, M., Tanimura, N., Iijima, S., Ishikawa, S., Kamasaki, T., Akieda, Y., Ishitani, T., Hayashi, T., Toda, S., Yokoyama, K., Lee, C.G., Usami, I., Inoue, H., Takigawa, I., Gauquelin, E., Sugimura, K., Hino, N., Fujita, Y. (2022) Calcium sparks enhance the tissue fluidity within epithelial layers and promote apical extrusion of transformed cells. Cell Reports. 40:111078
Suzuki, H., Aoki, K., Kurosawa, K., Imagawa, K., Ohto, T., Yamada, M., Takenouchi, T., Kosaki, K., Ishitani, T. (2022) De novo non-synonymous CTR9 variants are associated with motor delay and macrocephaly: human genetic and zebrafish experimental evidence. Human molecular genetics. 31(22):3846-3854
Haraoka, Y., Akieda, Y., Nagai, Y., Mogi, C., Ishitani, T. (2022) Zebrafish imaging reveals TP53 mutation switching oncogene-induced senescence from suppressor to driver in primary tumorigenesis. Nature communications. 13:1417
Lohani, S., Funato, Y., Akieda, Y., Mizutani, K., Takai, Y., Ishitani, T., Miki, H. (2021) A novel role of PRL in regulating epithelial cell density by inducing apoptosis at confluence. Journal of Cell Science. 135(2):
Yatsuka, H., Hada, K., Shiraishi, H., Umeda, R., Morisaki, I., Urushibata, H., Shimizu, N., Sebastian, W.A., Hikida, T., Ishitani, T., Hanada, R., Shimada, T., Kimoto, K., Kubota, T., Hanada, T. (2021) Corrigendum to "Exosc2 deficiency leads to developmental disorders by causing a nucleotide pool imbalance in zebrafish". Biochemical and Biophysical Research Communications. 546:200
Zarate, Y.A., Uehara, T., Abe, K., Oginuma, M., Harako, S., Ishitani, S., Lehesjoki, A.E., Bierhals, T., Kloth, K., Ehmke, N., Horn, D., Holtgrewe, M., Anderson, K., Viskochil, D., Edgar-Zarate, C.L., Sacoto, M.J.G., Schnur, R.E., Morrow, M.M., Sanchez-Valle, A., Pappas, J., Rabin, R., Muona, M., Anttonen, A.K., Platzer, K., Luppe, J., Gburek-Augustat, J., Kaname, T., Okamoto, N., Mizuno, S., Kaido, Y., Ohkuma, Y., Hirose, Y., Ishitani, T., Kosaki, K. (2021) CDK19-related disorder results from both loss-of-function and gain-of-function de novo missense variants. Genetics in medicine : official journal of the American College of Medical Genetics. 23(6):1050-1057
Uehara, T., Abe, K., Oginuma, M., Ishitani, S., Yoshihashi, H., Okamoto, N., Takenouchi, T., Kosaki, K., Ishitani, T. (2020) Pathogenesis of CDK8-associated disorder: two patients with novel CDK8 variants and in vitro and in vivo functional analyses of the variants. Scientific Reports. 10:17575
Tsukiyama, T., Zou, J., Kim, J., Ogamino, S., Shino, Y., Masuda, T., Merenda, A., Matsumoto, M., Fujioka, Y., Hirose, T., Terai, S., Takahashi, H., Ishitani, T., Nakayama, K.I., Ohba, Y., Koo, B.K., Hatakeyama, S. (2020) A phospho-switch controls RNF43-mediated degradation of Wnt receptors to suppress tumorigenesis. Nature communications. 11:4586
Inoue, M., Hada, K., Shiraishi, H., Yatsuka, H., Fujinami, H., Morisaki, I., Nishida, Y., Matsubara, E., Ishitani, T., Hanada, R., Matsumoto, M., Penninger, J.M., Ihara, K., Hanada, T. (2020) Tyrosine pre-transfer RNA fragments are linked to p53-dependent neuronal cell death via PKM2. Biochemical and Biophysical Research Communications. 525(3):726-732
Takeuchi, Y., Narumi, R., Akiyama, R., Vitiello, E., Shirai, T., Tanimura, N., Kuromiya, K., Ishikawa, S., Kajita, M., Tada, M., Haraoka, Y., Akieda, Y., Ishitani, T., Fujioka, Y., Ohba, Y., Yamada, S., Hosokawa, Y., Toyama, Y., Matsui, T., Fujita, Y. (2020) Calcium Wave Promotes Cell Extrusion. Current biology : CB. 30(4):670-681.e6
Akieda, Y., Ogamino, S., Furuie, H., Ishitani, S., Akiyoshi, R., Nogami, J., Masuda, T., Shimizu, N., Ohkawa, Y., Ishitani, T. (2019) Cell competition corrects noisy Wnt morphogen gradients to achieve robust patterning in the zebrafish embryo. Nature communications. 10:4710
Abe, K., Shimada, A., Tayama, S., Nishikawa, H., Kaneko, T., Tsuda, S., Karaiwa, A., Matsui, T., Ishitani, T., Takeda, H. (2019) Horizontal Boundary Cells, a Special Group of Somitic Cells, Play Crucial Roles in the Formation of Dorsoventral Compartments in Teleost Somite. Cell Reports. 27:928-939.e4
Ueda, Y., Shimizu, Y., Shimizu, N., Ishitani, T., Ohshima, T. (2018) Involvement of Sonic hedgehog and Notch signaling in regenerative neurogenesis in adult zebrafish optic tectum after stab injury. The Journal of comparative neurology. 526(15):2360-2372
Kulkeaw, K., Inoue, T., Ishitani, T., Nakanishi, Y., Zon, L.I., Sugiyama, D. (2017) Purification of zebrafish erythrocytes as a means of identifying a novel regulator of haematopoiesis. British journal of haematology. 180(3):420-431
Leong, K.H., Mahdzir, M.A., Din, M.F., Awang, K., Tanaka, Y., Kulkeaw, K., Ishitani, T., Sugiyama, D. (2017) Induction of intrinsic apoptosis in leukaemia stem cells and in vivo zebrafish model by betulonic acid isolated from Walsura pinnata Hassk (Meliaceae). Phytomedicine : international journal of phytotherapy and phytopharmacology. 26:11-21
Okuno, T., Ishitani, T., Yokomizo, T. (2015) Biochemical Characterization of Three BLT Receptors in Zebrafish. PLoS One. 10:e0117888
Saita, S., Shirane, M., Ishitani, T., Shimizu, N., Nakayama, K.I. (2014) Role of the ANKMY2-FKBP38 Axis in Regulation of the Sonic Hedgehog (Shh) Signaling Pathway. The Journal of biological chemistry. 289(37):25639-54
Shimizu, N., Ishitani, S., Sato, A., Shibuya, H., Ishitani, T. (2014) Hipk2 and PP1c Cooperate to Maintain Dvl Protein Levels Required for Wnt Signal Transduction. Cell Reports. 8(5):1391-404
Delgado, E.R., Yang, J., So, J., Leimgruber, S., Kahn, M., Ishitani, T., Shin, D., Wilson, G.M., Monga, S.P. (2014) Identification and Characterization of a Novel Small-Molecule Inhibitor of beta-Catenin Signaling. The American journal of pathology. 184(7):2111-22
Wada, H., Ghysen, A., Asakawa, K., Abe, G., Ishitani, T., and Kawakami, K. (2013) Wnt/Dkk Negative Feedback Regulates Sensory Organ Size in Zebrafish. Current biology : CB. 23(16):1559-65
Shimizu, N., Kawakami, K., and Ishitani, T. (2012) Visualization and exploration of Tcf/Lef function using a highly responsive Wnt/beta-catenin signaling-reporter transgenic zebrafish. Developmental Biology. 370(1):71-85
Ota, S., Ishitani, S., Shimizu, N., Matsumoto, K., Itoh, M., and Ishitani, T. (2012) NLK positively regulates Wnt/beta-catenin signalling by phosphorylating LEF1 in neural progenitor cells. The EMBO journal. 31(8):1904-1915
Kulkeaw, K., Ishitani, T., Kanemaru, T., Ivanovski, O., Nakagawa, M., Mizuochi, C., Horio, Y., and Sugiyama, D. (2011) Cold exposure down-regulates zebrafish pigmentation. Genes to cells : devoted to molecular & cellular mechanisms. 16(4):358-367
Yamamoto, M., Morita, R., Mizoguchi, T., Matsuo, H., Isoda, M., Ishitani, T., Chitnis, A.B., Matsumoto, K., Crump, J.G., Hozumi, K., Yonemura, S., Kawakami, K., and Itoh, M. (2010) Mib-Jag1-Notch signalling regulates patterning and structural roles of the notochord by controlling cell-fate decisions. Development (Cambridge, England). 137(15):2527-2537
Sugiyama, K., Nishide, K., Matsuo, H., Okigawa, S., Okano, M., Ishitani, T., Matsumoto, K., and Itoh, M. (2010) Delta1 family members are involved in filopodial actin formation and neuronal cell migration independent of Notch signaling. Biochemical and Biophysical Research Communications. 398(1):118-124
Kulkeaw, K., Ishitani, T., Kanemaru, T., Fucharoen, S., and Sugiyama, D. (2010) Cold exposure down-regulates zebrafish hematopoiesis. Biochemical and Biophysical Research Communications. 394(4):859-864
Ishitani, T., Hirao, T., Suzuki, M., Isoda, M., Ishitani, S., Harigaya, K., Kitagawa, M., Matsumoto, K., and Itoh, M. (2010) Nemo-like kinase suppresses Notch signalling by interfering with formation of the Notch active transcriptional complex. Nature cell biology. 12(3):278-285
Tsukada, Y., Ishitani, T., and Nakayama, K.I. (2010) KDM7 is a dual demethylase for histone H3 Lys 9 and Lys 27 and functions in brain development. Genes & Development. 24(5):432-437
Ivanovski, O., Kulkeaw, K., Nakagawa, M., Sasaki, T., Mizuochi, Ch., Horio, Y., Ishitani, T., and Sugiyama, D. (2009) Characterization of kidney marrow in Zebrafish (Danio Rerio) by using a new surgical technique. Prilozi. 30(2):71-80
Ishitani, T., Matsumoto, K., Chitnis, A.B., and Itoh, M. (2005) Nrarp functions to modulate neural-crest-cell differentiation by regulating LEF1 protein stability. Nature cell biology. 7(11):1106-1112