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Research
General Information
ZIRC
ZFIN ID: ZDB-LAB-040127-1
Arindam Majumdar Lab
PI/Director: Majumdar, Arindam
Contact Person: Majumdar, Arindam
Email: arindam.majumdar@genpat.uu.se
URL:
Address: Department of Genetics and Pathology Rudbecklaboratoriet Uppsala University Dag Hammarskjölds väg 20 751 85 Uppsala Sweden
Country: Sweden
Phone:
Fax:
Line Designation: None assigned


GENOMIC FEATURES ORIGINATING FROM THIS LABNo data available


STATEMENT OF RESEARCH INTERESTS
My lab is using the zebrafish pronephros as a genetics and biomedical model system to identify the developmental signals that control vertebrate nephrogenesis and to understand how these signals become mis-regulated in disease states. Major areas of research include cell fate, cell-cell communication, epithelial polarity, tubulogenesis, podocyte differentiation and glomerular function.

Recently, we have applied the zebrafish pronephros as an experimental system to conduct a novel, in vivo functional screen for genes required for glomerular filtration. We have coupled morpholino knockdown with a physiological glomerular filtration assay that can be performed in living zebrafish larvae. Using this screen, we have identified several genes which are required for the selective permeability of the glomerular filtration barrier, a major target in human glomerular pathologies. The screen has identified new genes and signaling pathways that are currently under study.


LAB MEMBERS


ZEBRAFISH PUBLICATIONS OF LAB MEMBERS x
Hildebrand, S., Hultin, S., Subramani, A., Petropoulos, S., Zhang, Y., Cao, X., Mpindi, J., Kalloniemi, O., Johansson, S., Majumdar, A., Lanner, F., Holmgren, L. (2017) The E-cadherin/AmotL2 complex organizes actin filaments required for epithelial hexagonal packing and blastocyst hatching. Scientific Reports. 7:9540
Hultin, S., Subramani, A., Hildebrand, S., Zheng, Y., Majumdar, A., Holmgren, L. (2017) AmotL2 integrates polarity and junctional cues to modulate cell shape. Scientific Reports. 7:7548
Abu-Siniyeh, A., Owen, D.M., Benzing, C., Rinkwitz, S., Becker, T.S., Majumdar, A., Gaus, K. (2016) The aPKC/Par3/Par6 polarity complex and membrane order are functionally inter-dependent in epithelia during vertebrate organogenesis. Traffic (Copenhagen, Denmark). 17(1):66-79
Ebarasi, L., Ashraf, S., Bierzynska, A., Gee, H.Y., McCarthy, H.J., Lovric, S., Sadowski, C.E., Pabst, W., Vega-Warner, V., Fang, H., Koziell, A., Simpson, M.A., Dursun, I., Serdaroglu, E., Levy, S., Saleem, M.A., Hildebrandt, F., Majumdar, A. (2015) Defects of CRB2 Cause Steroid-Resistant Nephrotic Syndrome. American journal of human genetics. 96(1):153-61
Hultin, S., Zheng, Y., Mojallal, M., Vertuani, S., Gentili, C., Balland, M., Milloud, R., Belting, H.G., Affolter, M., Helker, C.S., Adams, R.H., Herzog, W., Uhlen, P., Majumdar, A., Holmgren, L. (2014) AmotL2 links VE-cadherin to contractile actin fibres necessary for aortic lumen expansion. Nature communications. 5:3743
Hayashi, M., Majumdar, A., Li, X., Adler, J., Sun, Z., Vertuani, S., Hellberg, C., Mellberg, S., Koch, S., Dimberg, A., Young Koh, G., Dejana, E., Belting, H.G., Affolter, M., Thurston, G., Holmgren, L., Vestweber, D., and Claesson-Welsh, L. (2013) VE-PTP regulates VEGFR2 activity in stalk cells to establish endothelial cell polarity and lumen formation. Nature communications. 4:1672
Owen, D.M., Magenau, A., Majumdar, A., and Gaus, K. (2010) Imaging membrane lipid order in whole, living vertebrate organisms. Biophysical journal. 99(1):L7-L9
Ebarasi, L., He, L., Hultenby, K., Takemoto, M., Betsholtz, C., Tryggvason, K., and Majumdar, A. (2009) A reverse genetic screen in the zebrafish identifies crb2b as a regulator of the glomerular filtration barrier. Developmental Biology. 334(1):1-9
Zheng, Y., Vertuani, S., Nyström, S., Audebert, S., Meijer, I., Tegnebratt, T., Borg, J.P., Uhlén, P., Majumdar, A., and Holmgren, L. (2009) Angiomotin-Like Protein 1 Controls Endothelial Polarity and Junction Stability During Sprouting Angiogenesis. Circulation research. 105(3):260-270
Sekine, Y., Nishibori, Y., Akimoto, Y., Kudo, A., Ito, N., Fukuhara, D., Kurayama, R., Higashihara, E., Babu, E., Kanai, Y., Asanuma, K., Nagata, M., Majumdar, A., Tryggvason, K., and Yan, K. (2009) Amino Acid Transporter LAT3 Is Required for Podocyte Development and Function. Journal of the American Society of Nephrology : JASN. 20(7):1586-1596
Vasilyev, A., Liu, Y., Mudumana, S., Mangos, S., Lam, P.Y., Majumdar, A., Zhao, J., Poon, K.L., Kondrychyn, I., Korzh, V., and Drummond, I.A. (2009) Collective Cell Migration Drives Morphogenesis of the Kidney Nephron. PLoS Biology. 7(1):e9
Ernkvist, M., Luna Persson, N., Audebert, S., Lecine, P., Sinha, I., Liu, M., Schlueter, M., Horowitz, A., Aase, K., Weide, T., Borg, J.P., Majumdar, A., and Holmgren, L. (2009) The Amot/Patj/Syx signaling complex spatially controls RhoA GTPase activity in migrating endothelial cells. Blood. 113(1):244-253
Raschperger, E., Neve, E.P., Wernerson, A., Hultenby, K., Pettersson, R.F., and Majumdar, A. (2008) The coxsackie and adenovirus receptor (CAR) is required for renal epithelial differentiation within the zebrafish pronephros. Developmental Biology. 313(1):455-464
Aase, K., Ernkvist, M., Ebarasi, L., Jakobsson, L., Majumdar, A., Yi, C., Birot, O., Ming, Y., Kvanta, A., Edholm, D., Aspenström, P., Kissil, J., Claesson-Welsh, L., Shimono, A., and Holmgren, L. (2007) Angiomotin regulates endothelial cell migration during embryonic angiogenesis. Genes & Development. 21(16):2055-2068
Majumdar, A. and Drummond, I.A. (2000) The zebrafish floating head mutant demonstrates podocytes play an important role in directing glomerular differentiation. Developmental Biology. 222(1):147-157
Majumdar, A., Lun, K., Brand, M., and Drummond, I.A. (2000) Zebrafish no isthmus reveals a role for pax2.1 in tubule differentiation and patterning events in the pronephric primordia. Development (Cambridge, England). 127(10):2089-2098
Liu, A., Majumdar, A., Schauerte, H.E., Haffter, P., and Drummond, I.A. (2000) Zebrafish wnt4b expression in the floor plate is altered in sonic hedgehog and gli-2 mutants. Mechanisms of Development. 91(1-2):409-413
Majumdar, A. and Drummond, I.A. (1999) Podocyte differentiation in the absence of endothelial cells as revealed in the zebrafish avascular mutant, cloche. Developmental genetics. 24(3-4):220-229
Drummond, I.A., Majumdar, A., Hentschel, H., Elger, M., Solnica-Krezel, L., Schier, A.F., Neuhauss, S.C., Stemple, D.L., Zwartkruis, F., Rangini, Z., Driever, W., and Fishman, M.C. (1998) Early development of the zebrafish pronephros and analysis of mutations affecting pronephric function. Development (Cambridge, England). 125:4655-4667