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ZDB-IMAGE-210422-18

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ZDB-IMAGE-210422-18
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Figures for Sutton et al., 2021
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Figure Caption

The role of peptidoglycan metabolism components and macrophages in <italic>S</italic>. <italic>aureus</italic> host-pathogen interactions.

Mice (n = 10) were injected with approximately 1 x 105 CFU of NewHG kanR (SJF 3680), NewHG pbp4::ery (SJF 5103) or NewHG sagB::kan (SJF 4912) 24 hours post treatment with either empty liposomes or clodronate containing liposomes. 72 hpi, mice were sacrificed and the weight change (A, E, * p = 0.0254) and liver (B, F, * p = 0.0243), kidney (C, G, ** p = 0.0030) and spleen (D, H, * p = 0.0196) CFU were determined. (NewHG kanR–black circles, NewHG pbp4::ery—blue squares, NewHG sagB::kan- red squares). One mouse in the NewHG kanR clodronate treated group and the NewHG pbp4 clodronate treated groups was culled at 48 hpi due to reaching severity limits and have been excluded from analysis. MDMs were infected with S. aureus NewHG kanR (SJF 3680, black bars), (I) NewHG pbp4::ery (SJF 5103, blue bars) or (J) or NewHG sagB::kan (SJF 4912, red bars) at a MOI of 5 (1 x 106 CFU) for 4 hours before being treated with gentamycin for 0.5 hours to kill extracellular bacteria. MDMs were lysed at specific time points and intracellular bacterial numbers were determined. Paired two-tailed t-tests were used to compare between the strain CFU at subsequent time points. (For (I) *** p = 0.0008 and for (J) * p = 0.0250, ** p = 0.0075, *** p = 0.0003) Error bars show ±SD. (n = 3, each consisting of 2 intra-assay repeats).

Acknowledgments
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