Fig 2

Fig 2

Representative muropeptide profiles of NewHG kan^R (SJF 3680) cultured in TSB in (A) exponential phase (OD₆₀₀ ~0.6) or (B) stationary phase (OD₆₀₀ ~9). Muropeptides have been labelled after being identified by MS (S1 Table) (C) Representative muropeptide profile of NewHG kan^R (SJF 3680) recovered from murine kidneys 72 hpi. Identified muropeptides are labelled using muropeptide numbers from S3 Table. (D) Area of eluted identified UV-absorbing peaks shown as a percentage of the total of all identified peaks grouped based on cross-linking (exponential phase TSB—black bars n = 3, stationary phase TSB–blue bars n = 3, NewHG kan^R recovered from murine kidney–red bars, n = 2 independent experiments, each consisting of 10 mice, error bars represent the standard deviation of the mean). A two-way ANOVA with Sidak’s multiple comparison post-test was used to compare abundance of each muropeptide species (monomers * p = 0.0108, ** p = 0.0081; oligomers * p = 0.0101, *** p = 0.0002). Representative generic muropeptide structures, (E) monomeric, (F) dimeric and (G) oligomeric.