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Fig. S4 Pilocarpine-induced seizures exhibit features similar to pentylenetetrazole (PTZ)-induced seizures, but the ictogenesis occurs less rapidly A) An optical section of a zebrafish larva expressing GCaMP6s in all neurons, obtained by two-photon microscopy, dorsal view (left). Individual neurons (right) in color-coded brain regions: telencephalon (blue), thalamus (red), and optic tectum (green). White bar reflects 50 μm. B) Activity of individual neurons (ΔF/F) over time, organized by brain region. Application of 60 mM pilocarpine was done at the beginning of the recording. Baseline is indicated for a time period preceding strongly correlated activity. Warmer colors indicate stronger activity. C) Percentage of active neurons (>3stdbaseline) in the whole brain and per brain area during baseline (black), preictal (gray) and seizure (red) periods. D) Average activity of the active neurons, defined by the area under the curve of the ΔF/F trace, in the whole brain and per brain area. E) Average pairwise Pearson’s correlation across the whole brain, and within individual brain regions. F) Histogram representing the distribution of all correlation coefficients between neurons from all animals. G) Relation between pairwise Pearson’s correlation of neural activity and the distance between each neuron pair. Dotted lines represent the results when neuronal locations are shuffled. n = 6 fish. Shaded regions and error bars represent the s.e.m. (*p= <0.05, ns= not significant, Wilcoxon signed-rank test).