ZFIN Image: Yu et al., 2019, Fig. 3-S1

Image

Figure Caption

Fig. 3-S1

Glial and Non-glial cell clusters identification from the scRNA-seq data.

(A–A₂) Tg(gfap:GFP) (green) and PCNA (red) immunofluorescences show large-area injury induces many RG (GFP⁺/PCNA⁺ yellow cells, white arrows) to proliferate at 3 dpi. White dashed lines represent the optic tectum boundary. Yellows arrow heads indicate the injury sites. (B) t-SNE plot of 2298 single cells at 3 dpi revealing 15 cell clusters. Black dashed line indicates picked glial cell clusters (cluster 0, 3, 4, 7, 8, 9, 10). (C) Heatmap showing the expression of top 20 marker genes that characterize each cell clusters. Rows represent genes while columns represent cells. Black arrows indicate the picked glial cell clusters (cluster 0, 3, 4, 7, 8, 9, 10). (D) t-SNE plots showing expression of 9 genes utilized to identify neuronal clusters and glial clusters. (E) Violin plots of expression of 9 genes shown in (D). RG, radial glia; TeO, tectal opticum; PGZ, periventricular gray zone; TS, torus semicircularis.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife