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Fig. 1
col11a2 zebrafish mutant larvae show progressively altered type II collagen protein localization in jaw cartilage. (a,b) Maximum projection of ventral and lateral confocal image stacks from wt (a) and homozygous mutant (col11a2−/−) (b) larvae immunostained for type II collagen at three time points (3, 5 and 7 dpf). White arrows indicate areas of change in type II collagen distribution in the ECM. Dashed insets show single-stack images of regions with reduced deposition (white asterisks represent areas where type II collagen is maintained in mutant fish and red asterisks show fragments of type II collagen-positive material outside the main cartilage elements). Red arrows show interoperculomandibular (IOM) ligament. Scale bar, 100 µm.