ZFIN Image: Pinto et al., 2018, Fig. 1

Image

Figure Caption

Fig. 1

Generation and characterisation of the Tg(hsp70:HA-dmrt2a) line. a Transgenic construct design. b Heat-shock protocol: embryos were raised at 28 °C and the heat-shock performed for 30 min at 39 °C. c qPCR analysis showing that dmrt2a transcripts are present from bud-stage until 20-somite stage. d Southern blot hybridisation using a ³²P-labelled γ-crystallin probe showing F0 fish with multiple bands (C⁺, positive control), wildtype fish (C⁻, negative control), and F1 generation fish with only one copy of the transgene or multiple copies. e The HA-Dmrt2a protein is produced with the correct molecular weight (57 kDa). f The fusion protein HA-Dmrt2a (red) co-localises with the nucleus (blue). h: hours, min: minutes, ss: somite stage. Scale bar: 30 μm

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.