IMAGE

Fig. 3

ID
ZDB-IMAGE-180730-28
Source
Figures for Haesemeyer et al., 2018
Image
Figure Caption

Fig. 3

Motor Cells Can Be Separated According to Behavior and Stimulus Conditions

(A) Example behavioral regressors (black) and activity trace of one correlated cell. Top: Cell encoding all motor events in a plane (orange); middle: cell encoding left flicks in a plane (purple); bottom: cell encoding swims in a plane (brown). Numbers indicate correlation coefficient.

(B) Clustered heatmap of correlations of motor-cell activity with motor regressors, including the following events: All motor: all motor events of a given plane; Flicks: only flicks in a plane; Right/left flicks: only included right/left flicks; Swims: only swims included; Evoked motor: only motor events while the heat stimulus was on were included; Spontaneous motor: only motor events while the heat stimulus was off were included. Cells are only assigned to a more specialized motor cluster if the correlation to the specialized regressor is significantly higher than to the general regressor (p < 0.01, bootstrap hypothesis test). all-motor (AM): N = 5049 cells; flicks (F): N = 420; flick-right (FR): N = 319; flick-left (FL): N = 298; swims (S): N = 1338; evoked-motor (EVK): N = 950; spontaneous-motor (SPNT): N = 763. 30 fish.

(C and D) Fraction of motor correlated cells within selected brain regions. Color scale indicates percentage of motor-correlated cells within each region. Scale bars, 100 μm. Pl.: pallium, Hab.: habenula, ncMLF: nucleus of the medial longitudinal fascicle, Crb.: cerebellum, Ant. HB: anterior hindbrain. Grey cells indicate brain regions that were not segmented. (C) Dorsal view of the brain, anterior left, left side bottom; (D) side view of left hemisphere, anterior left, dorsal top.

(E) Quantification of percentage of motor correlated cells in select brain regions. Red bars: forebrain; purple: midbrain; blue: hindbrain.

(F) Distribution of flick-right (green) and flick-left (purple) cells, top projection. Anterior left, scale bar, 100 μm

G) Distribution of evoked-motor (red) and spontaneous-motor (blue) cells, top projection. Anterior left, scale bar, 100 μm.

Grey cells in (F)–(G) are non-motor-related cells representing brain outline.

See also Figure S3.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Neuron