ZFIN Image: König et al., 2017, Fig. 5

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Fig. 5

Elongated uninjured fins of alf^dty86 mutant fish contain extended actinotrichia. (A, B) Live-imaging of adult caudal fins in wild-type fish and alf^dty86 mutants. (C, D) Alcian blue histological staining of uninjured caudal fins shows uncalcified lepidotrichia/cartilage (blue). Bundles of actinotrichia are unstained, but visible by a differential contrast at the tips of rays. Brown patches correspond to endogenous fin pigmentation. N = 4 fins. (E, F) Double immunostaining of wild-type and alf^dty86 mutant fins using And1 (green) and osteoblast marker Zns5 (red) antibodies. Tissue autofluorescence shown in blue. N = 4 fins. (G, H) In-situ hybridization with and1 probe on whole-mount fins labels uninjured fin margin of alf^dty86 mutant fins, but not wild-type ones. N = 5 fins. (I) Quantification of length of actinotrichia in wild-type versus alf^dty86 uninjured fins based on And1 staining (6 rays quantified for each fin, N = 4 fish per group. Error bars indicate SEM, ** P<0.01).

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Acknowledgments

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Reprinted from Developmental Biology, 433(2), König, D., Page, L., Chassot, B., Jaźwińska, A., Dynamics of actinotrichia regeneration in the adult zebrafish fin, 416-432, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.