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Fig. 3
In vivo half-depth of field OPT reconstruction of a 5 days post fertilization transgenic mpx:GFP zebrafish, combining sequential fluorescence (for neutrophil GFP expression, shown in green) and transmission (for zebrafish morphology, shown in grey) acquisitions.
(a) Single slice through the reconstruction, accompanying (b) YZ cross-section (CS) along vertical yellow line in (a). (c) Maximum intensity projection (MIP) through entire reconstructed volume. (d) Magnified view of reconstruction within red box indicated in (b), and (e) line profile through neutrophils cells.
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