ZFIN Image: Naylor et al., 2016, Fig. 5

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Figure Caption

Fig. 5

Effects of HUA treatment on tubulogenesis and caudal migration.

(A) Left panel shows the PST region and the right panel shows the DL region of an embryo labelled with EdU (red) and Hnf1b (green) at the 24 hpf stage. (B) Transverse sections of Tg(cdh17:egfp) embryos treated with HUA and DMSO and stained with Phalloidin to label F-actin and DAPI to label nuclei. (C) Panels show lateral views of HUA and DMSO treated embryos double stained for cdh17 and uncaged fluorescein (lineage tracer) that was labelled in the intermediate mesoderm lateral to somite 8 at the 12-somite stage. (D) Oblique lateral views of embryos treated with HUA and DMSO (control) and analysed for pax2a, slc4a4, slc12a1 and slc12a3 transcripts (black arrowheads indicate the anteriormost position of the DL segment).

Acknowledgments

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