ZFIN Image: Xue et al., 2017, Fig. S5

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Figure Caption

Fig. S5

Screening of downstream targets of klf6a in the CHT. Related to Figure 5.

(A) The cartoon showing the migration and lodgement process of HSPC in the CHT of zebrafish. (B) qPCR results of chemokine ligands in control and klf6a morphants at 52 dpf. (mean ± SD, t-test, *P<0.05, **P<0.01, ***P<0.001, ns>0.05, n = 3). (C) Expression of cxcl12a, cxcl12b, tgfbr2 and vegfa in control and klf6a morphants. (D) qPCR result of ccl25b, cxcl8b and mmp9 in sorted kdrl⁺ cells of CHT at 52 dpf (mean ± SD, t-test, *P<0.05, ***P<0.001, n = 3). (E) Heat-map analysis of chemokine ligands and receptors expressed in ECs and HSPCs in the CHT region at 52 hpf. Black arrows indicate chemokine ligand and receptor which is enriched in ECs or HSPCs respectively. (F) qPCR results of ccl25b and ccr7 expression in CD41⁺ and kdrl⁺ sorted cells from the CHT at 52 hpf.

Acknowledgments

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Reprinted from Developmental Cell, 42(4), Xue, Y., Lv, J., Zhang, C., Wang, L., Ma, D., Liu, F., The Vascular Niche Regulates Hematopoietic Stem and Progenitor Cell Lodgment and Expansion via klf6a-ccl25b, 349-362.e4, Copyright (2017) with permission from Elsevier. Full text @ Dev. Cell