|ZFIN ID: ZDB-IMAGE-170922-44|
Ccl25b Acts Downstream of Klf6a to Facilitate HSPC Settlement and Expansion
(A) Time-lapse imaging of kdrl:mCherry/runx1:en-GFP in control and ccl25b morphants. The white arrowheads mark HSPCs and the white circles indicate HSPC pockets. Scale bar, 50 μm.
(B) The statistical data of runx1+ cells in D/M/V of CVP in control and ccl25b morphants at 50 hpf (mean ± SD, t test; ∗p < 0.05, ns [not significant] > 0.05, n = 8).
(C) Confocal imaging of kdrl:mCherry/runx1:en-GFP CHT at 3 dpf in control embryos and ccl25b morphants. Yellow arrowheads mark GFP+ cells. The white dashed lines mark the outlines of caudal artery and vein. Scale bar, 50 μm.
(D) The quantification of runx1:en-GFP+ cells shown in (C) (mean ± SD, t test; ∗∗p < 0.01, n = 8).
(E) Confocal imaging of FISH (cmyb)/immunofluorescence (PH3) at 60 hpf in CHT of control embryos and ccl25b morphants. White arrowheads mark double-positive cells. Scale bar, 50 μm.
(F) The statistical data showing that the percentage of double-positive/cmyb+ cells was reduced significantly in the morphants (mean ± SD, t test; ∗∗∗p < 0.001, n = 8).
(G) qPCR result of cell division-related gene expression in control and ccl25b morphants (mean ± SD, t test; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ns > 0.05, n = 3).
See also Figure S6.
|Acknowledgments:||ZFIN wishes to thank the journal for permission to reproduce figures from this article. Please note that this material may be protected by copyright.|