ZFIN ID: ZDB-IMAGE-170914-24
Figures for Nguyen-Chi et al., 2017

Gene:

Figure Caption/Comments:

Fig. 4

Depletion of late-recruited macrophages affects regeneration by impairing mesenchymal cell behavior but not cell death. (a and b) Representative images show cell death (green) in intact and amputated fin at (a) 48hpA and (b) 24hpA using confocal microscopy. (a) Cell death was detected using Acridine Orange staining in larvae in which all macrophages were depleted with L-clodronate treatment 24h before amputation (L-clodronate 1) or not (L-PBS). (b) Cell death was detected using Acridine Orange staining in larvae in which late macrophages were depleted with L-clodronate treatment at 6hpA (L-clodronate 2) or not (L-PBS). Dotted lines outline the fin, asterisks show autofluorescence of the pigments and arrowheads show dead cells. Scale bar=100μm. (c,d) Cell death counts in indicated conditions (Nlarvae=7–18 per group from two independent experiments, mean values±S.E.M., *P<0.05). (e) Tg(rcn3:gal4/UAS:Ds-Red) larvae were amputated at 3dpf and injected with either L-PBS or L-clodronate at 6hpA. Fin images are representative confocal maximum projections of Ds-Red (magenta) fluorescence in mesenchymal cells of the fins at 72hpA. Arrowheads show round mesenchymal cells at the wound of late macrophage-depleted larvae. Scale bar=100 μm

Figure Data:
Acknowledgments:
ZFIN wishes to thank the journal for permission to reproduce figures from this article. Please note that this material may be protected by copyright.