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Fig. S7
Mosaic CRISPR knockout of Wnt16 affects cell migration at the medial region of the jaw joint. (A-B'): Tg(Sox10:GAL4-VP16) and Tg(UAS:Kaede) transgenic line drives expression of kaede protein (green) in cartilage of control injected (A), and mosaic CRISPR Wnt16 knockout (B) zebrafish. Maximum projections of the jaw joint from stacks of tiff images (A,B) and single slice/substacks through the same jaw joint to show cell morphology (A',B') are represented. At the jaw joint, medially located kaede expressing cells are photoconverted to red kaede at 3dpf (left panels). Right panels show jaw joints from the same larva reimaged at 5dpf. Photoconverted cells appear red/orange due to presence of photoconverted red kaede and expression of newly made green kaede protein under control of sox10 promoter. (C): Percentage increase in total area of cells expressing photoconverted red kaede between 3 and 5dpf in control injected (Fig. 4E) and CRISPR Wnt16 knockout zebrafish jaw joints. (n=10, 22 joints). Kruskal-Wallis tests were performed for (C). ***=p≤0.001. Bars on graph represent mean and 95%CI.