ZFIN Image: Zhang et al., 2016, Fig. 6

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Figure Caption

Fig. 6

Gap junctions between BCs are necessary for the occurrence of BC waves.

(a) Confocal images showing tracer coupling among nearby BCs. Neurobiotin was introduced into the BC soma of a 3-d.p.f. Tg(Gal4-VP16^xfz43,UAS:nfsB-mCherry) larva through a recording micropipette. Due to the small size of zebrafish BCs, the soma of the recorded BC was pulled away by the removal of the micropipette. White arrows and arrowheads indicate the somata and ATs of coupled BCs. The white asterisks indicate two BCs with no expression of mCherry, because not all BCs express mCherry in Tg(Gal4-VP16^xfz43,UAS:nfsB-mCherry) larvae. Scale bar, 10 μm. (b) Summary of data showing the number of coupled BCs. (c) Example showing spontaneous calcium activities of BCs before and 10 min after bath application of FFA (50 μM). (d) Summary of data showing the effects of FFA (50 μM) and 18α-GA (50 μM) on BC waves. Data obtained from the same larva are connected by a line. *P<0.05, **P<0.01; two-tailed paired Student’s t-test for the data in d. Data are represented as mean±s.e.m.

Acknowledgments

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