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Fig. 1

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ZDB-IMAGE-170517-31
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Figures for Anelli et al., 2017
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Fig. 1

BRAFV600E expression in thyrocytes disrupts follicle structure.

(A) Schematic diagram of the constructs used to create transgenic lines, containing a specific zebrafish thyroglobulin promoter (zf tg prom) driving expression of either human BRAFV600E or TdTomato fluorophore. (B–C) Brightfield micrographs overlayed on to an epifluorescent micrograph of a live 5 dpf tg-TOM and tg-BRAFV600E-TOM larvae. (D–E) Representative confocal images of 5 dpf fixed tg-TOM and tg-BRAFV600E-TOM larvae immunostained with anti-T4 antibody; white arrows indicate T4-positive follicles. (F) Quantification of the total follicle number in tg-TOM and tg-BRAFV600E-TOM larvae (n = 20). (G) Measurement of follicle diameter in tg-TOM and tg-BRAFV600E-TOM (n = 45 and n = 30, respectively). ***p≤0.001, Student's two-tailed t-test.

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